| FUT2/01基因座在中国北方汉族人群中的多态性 |
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| 引用本文： | 田小菲,李忠杰,王保捷,丁梅,宣金锋,邢佳鑫,李春梅,庞灏. FUT2/01基因座在中国北方汉族人群中的多态性[J]. 法医学杂志, 2009, 25(5): 345-347. DOI: 10.3969/j.issn.1004-5619.2009.05.008 |
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| 作者姓名： | 田小菲 李忠杰 王保捷 丁梅 宣金锋 邢佳鑫 李春梅 庞灏 |
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| 作者单位： | 中国医科大学法医学院法医血清教研室,辽宁,沈阳,110001 |
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| 摘 要： | 目的调查STR基因座FUT2/01在中国人群中的基因结构特征及其在中国人群中的多态性分布特点,并对其法医学应用前景进行探讨。方法应用PCR方法扩增FUT2/01基因,DNA测序确定所有等位基因的序列;常规聚丙烯酰胺凝胶电泳调查中国人群中FUT2/01基因座的多态性分布,同时应用荧光标记引物进行自动化检测分析和基因型鉴定。结果DNA测序结果仅显示核心序列的重复数目变化所导致的长度差异;在所调查的中国汉族人群162例个体中共发现9种等位基因和28种基因型,系统的个体识别率为0.9639,父权排除率为0.6266。此外,荧光标记引物经自动化检测可对该基因座进行良好的分型。结论FUT2/01基因座在中国汉族人群中表现出高度的杂合性和个体特异性,在法医学鉴定中具有较高的应用价值。
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| 关 键 词： | 法医遗传学 多态现象,遗传 STR FUT2/01 汉族 |
Polymorphic Distribution of FUT2/01 in Northern Han Chinese Population |
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| TIAN Xiao-fei,LI Zhong-jie,WANG Bao-jie,DING Mei,XUAN Jin-feng,XING Jia-xin,LI Chun-mei,PANG Hao. Polymorphic Distribution of FUT2/01 in Northern Han Chinese Population[J]. Journal of Forensic Medicine, 2009, 25(5): 345-347. DOI: 10.3969/j.issn.1004-5619.2009.05.008 |
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| Authors: | TIAN Xiao-fei LI Zhong-jie WANG Bao-jie DING Mei XUAN Jin-feng XING Jia-xin LI Chun-mei PANG Hao |
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| Affiliation: | TIAN Xiao-fei,LI Zhong-jie,WANG Bao-jie,DING Mei,XUAN Jin-feng,XING Jia-xin,LI Chun-mei,PANG Hao(Department of Forensic Serology,School of Forensic Medicine,China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective To investigate the sequence features of FUT2/01 locus and its polymorphic distribu-tion in Chinese population,and to discuss its application potential in forensic medicine.Methods The al-leles on FUT 2/01 locus were amplified by PCR and then were sequenced.Furthermore,polymorphic dis-tribution of the locus was analyzed by polyacrylamide gel electrophoresis.The genotypes were characterized with fluorescence labeling followed by automatic detection system.Results The sequencing results only showed t... |
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| Keywords: | STR FUT2/01 |
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