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| DHPLC检测人体线粒体DNA异质性研究 | |
| 引用本文： | 高林林,严江伟,唐晖,任贺,王静,刘雅诚. DHPLC检测人体线粒体DNA异质性研究[J]. 刑事技术, 2006, 0(6): 27-30 |
| 作者姓名： | 高林林 严江伟 唐晖 任贺 王静 刘雅诚 |
| 作者单位： | 1. 浙江省杭州市公安局,310002 2. 北京市公安局法医检验鉴定中心,100085 3. 北京警察学院,100026 |
| 摘 要： | 目的建立筛选线粒体DNA异质性的DHPLC方法;检测线粒体DNA高变区的异质性频率。方法选取尸体18例,分别提取血、心、肝、脾、肺、肾、胰腺、脑、肌肉、皮肤、肋骨、指甲及毛发的mtDNA,用DHPLC筛选异质型样本,并用直接测序法进行验证。结果9例个体存在异质性,肌肉组织出现的异质性频率最高。结论正确认识线粒体DNA异质性对于法医学应用领域具有指导意义。 |
| 关 键 词： | 线粒体DNA 异质性 直接测序法 法医物证学 |
| 文章编号： | 1008-3650(2006)06-0027-04 |
| 修稿时间： | 2006-06-10 |
Analysis heteroplasmy in hypervariable region of human mtDNA within multiple tissues by DHPLC |
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| GAO Lin-lin,YAN Jiang-wei,TANG Hui,et al.. Analysis heteroplasmy in hypervariable region of human mtDNA within multiple tissues by DHPLC[J]. Forensic Science and Technology, 2006, 0(6): 27-30 | |
| Authors: | GAO Lin-lin YAN Jiang-wei TANG Hui et al. |
| Abstract: | Objective To establish the method of screening mtDNA heteroplasmy by DHPLC,and to investigate the frequency of mtDNA heteroplasmy in control region within multiple tissues of the same individual, it is provided reference by use of mtDNA technology in forensic community.Method Select blood,heart,liver,spleen,lung,kidney,pancreas,brain,muscle,skin,rib,nail and hair of 18 individuals,screening the heteroplasmyic samples by DHPLC,and confirm by direct sequencing.Result 9 heteroplasmic individuals were detected,the frequency of heteroplasmy is highest in muscle tissue.Conclusion It is important to take into account mtDNA heteroplasmy in forensic applied field. |
| Keywords: | DHPLC |
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