|
|||||
|
|
| 利用aroA基因建立鸡传染性鼻炎PCR诊断方法 | |
| 引用本文： | 宋敏训,陈小玲,牟建青,T.Terry,王莉莉,李锋,艾武,张秀美. 利用aroA基因建立鸡传染性鼻炎PCR诊断方法[J]. 中国兽医科学, 2001, 31(10): 7-9 |
| 作者姓名： | 宋敏训 陈小玲 牟建青 T.Terry 王莉莉 李锋 艾武 张秀美 |
| 作者单位： | 1. 山东省农业科学院家禽研究所,山东,济南,250023 2. 北京市农林科学院畜牧兽医研究所,北京,100081 3. 澳大利亚昆士兰大学微生物系 |
| 摘 要： | 利用aroA基因设计了1对引物,分别对10株标准副鸡嗜血杆菌(HPG)菌株、14株分离的HPG菌株进行PCR扩增,结果均得到了与预期大小一致的片段,而对10株非HPG菌株和3株病毒进行扩增则无相应片段产生;该PCR能检测出10个菌细胞.与常规PCR方法相比,aroA-PCR的敏感性更高. |
| 关 键 词： | aroA基因 鸡传染性鼻炎 聚合酶链反应 |
| 文章编号： | 1000-6419(2001)10-0007-03 |
| 修稿时间： | 2001-04-02 |
Development of PCR Assay for Identification of Chicken Infectious Coryza Using HPG aroA Gene |
|
| SONG Min xun ,CHEN Xiao ling ,MU Jian qing ,Terry T ,WANG Li li ,LI Feng ,AI Wu ,ZNANG Xiu mei. Development of PCR Assay for Identification of Chicken Infectious Coryza Using HPG aroA Gene[J]. Chinese Veterinary Science, 2001, 31(10): 7-9 | |
| Authors: | SONG Min xun CHEN Xiao ling MU Jian qing Terry T WANG Li li LI Feng AI Wu ZNANG Xiu mei |
| Affiliation: | SONG Min xun 1,CHEN Xiao ling 2,MU Jian qing 1,Terry T 3,WANG Li li 1,LI Feng 1,AI Wu 1,ZNANG Xiu mei 1 |
| Abstract: | Using aroA gene of Haemopilus paragallinarum(HPG), the method of polymerase chain reaction(PCR)for identification of chicken infectious coryza was developed.24 HPG strains,10 non-HPG stains were tested respectively.The results showed sensitivity of the method for HPG was higher than traditional PCR,and it was consistent with the isolation and culture technique to results of detecting HPG from artificially infected swab samples of chickens. |
| Keywords: | aroA gene chicken infectious coryza PCR |
| 本文献已被 万方数据 等数据库收录！ | |