| 轮状病毒VP4-ST融合基因转化小球藻的筛选 |
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| 引用本文： | 任雪艳,王恒强,祝建波,孔庆军. 轮状病毒VP4-ST融合基因转化小球藻的筛选[J]. 中国兽医科学, 2010, 40(1) |
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| 作者姓名： | 任雪艳 王恒强 祝建波 孔庆军 |
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| 作者单位： | 任雪艳(石河子大学生命科学学院,新疆,石河子,832003;浙江大学生物系统工程与食品科学学院,浙江,杭州,310029);王恒强,祝建波,孔庆军(石河子大学生命科学学院,新疆,石河子,832003) |
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| 基金项目： | 石河子大学高层次人才启动基金项目 |
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| 摘 要： | 进行了椭圆小球藻对G418和卡那霉素的抗生素敏感性试验,同时采用电激转化法将构建好的含35S启动子的重组植物表达载体pBin-VP4-ST转化椭圆小球藻,并对转基因小球藻进行了PCR和PCR-Southern-blot检测。结果显示,30mg/L的G418为转化子的最佳抗生素筛选浓度。PCR和PCR-South-ern-blot检测结果显示,VP4-ST已成功插入到转基因小球藻基因组中,从而为用转基因小球藻作为生物反应器生产抗腹泻口服疫苗奠定了基础。
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| 关 键 词： | 小球藻 电激转化 聚合酶链反应 琼脂糖凝胶电泳 |
Selection of chlorella transformed with rotavirus VP4-ST fusion gene |
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| REN Xue-yan,,WANG Heng-qiang,ZHU Jian-bo,KONG Qing-jun. Selection of chlorella transformed with rotavirus VP4-ST fusion gene[J]. Chinese Veterinary Science, 2010, 40(1) |
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| Authors: | REN Xue-yan WANG Heng-qiang ZHU Jian-bo KONG Qing-jun |
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| Affiliation: | REN Xue-yan1,2,WANG Heng-qiang1,ZHU Jian-bo1,KONG Qing-jun1(1.College of Life Sciences,Shihezi University,Shihezi 832003,China,2.College of Biosystems Engineering , Food Science,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | The sensitivity tests of Chlorella ellipsoidea against kanamycin and G418 were conducted,and the re-constructed vector pBin-VP4-ST was transformed into chlorella through electroporation.The transgenic chlorellas were identified by PCR and PCR-Southern-blot.The results showed that the optimal selection condition of transformed chlorella was G418 30mg/L,and the positive transformed chlorella of the VP4-ST fusion gene was obtained.These results established a foundation for using transgenic chlorella as an effi... |
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| Keywords: | chlorella electroporation PCR agarose gel electrophoresis |
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