心肌炎猝死者心、脾中柯萨奇 B3病毒基因的检测

目的检测病毒性心肌炎（ viral myocarditis,VMC）猝死者心、脾组织柯萨奇 B3病毒 (CVB3)基因,探索 VMC的病原学诊断方法。方法运用原位逆转录 PCR技术检测实验组（ VMC猝死, 8例）及对照组（非心性死亡, 4例）心、脾组织中的 CVB3基因。结果实验组中, 3例（第 1, 4, 7例）心肌 CVB3基因阳性, 4例（第 2, 4 , 6, 7例）脾组织 CVB3基因阳性;对照组心、脾 CVB3基因均为阴性。结论心、脾组织中 CVB3基因联合阳性可能是 VMC的重要特征;联合检测心、脾中 CVB3基因可提高 VMC的病原学诊断率。

Detection of coxsackie virus B3 gene in myocardium and spleen samples in myocarditis with sudden death

OBJECTIVE: To detect the Coxsackie virus B3(CVB3) gene in myocardium and spleen tissues in viral myocarditis(VMC) with sudden death and to explore the diagnostic method for VMC by means of seeking pathogene. METHODS: By in situ RT-PCR, the detection of CVB3 gene in myocardium and spleen sections were performed in sudden death group caused by VMC and non-cardiac death group. RESULTS: In VMC group, CVB3 gene-positive signals were seen in myocardium sections(3 out of total 8 cases, No. 1, 4, 7 cases) and spleen sections(4 out of total 8 cases, No. 2, 4, 6, 7 cases). In non-cardiac death group, no positive signals were detected in both myocardium and spleen tissues. CONCLUSION: Positive detection of CVB3 gene in both myocardium and spleen maybe an important character of VMC and can improve the detecting pathogene in diagnosing VMC.