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| 乳牛前脂肪细胞原代培养过程中ADPN基因和HSL基因表达水平的检测 | |
| 引用本文： | 张辉,常维毅,张才,宋文华,王哲. 乳牛前脂肪细胞原代培养过程中ADPN基因和HSL基因表达水平的检测[J]. 中国兽医科学, 2007, 37(6): 510-514 |
| 作者姓名： | 张辉 常维毅 张才 宋文华 王哲 |
| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062;吉林农业科技学院,动物科学系,吉林,吉林,132109 2. 吉林农业科技学院,动物科学系,吉林,吉林,132109 3. 吉林大学,畜牧兽医学院,吉林,长春,130062 4. 吉林农业大学,动物科技学院,吉林,长春,130118 |
| 摘 要： | 选取犊牛大网膜脂肪组织,进行牛前脂肪细胞的单层贴壁培养,在细胞接种后第4、6、8、10、12、141、6 d分别提取细胞总RNA,采用实时荧光定量RT-PCR方法,检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。结果表明,单层培养的脂肪细胞在第4、6、8、10、12、141、6 d,ADPN基因mRNA的表达水平呈先下降后升高的趋势,而HSL基因mRNA的表达水平则呈下降趋势。 |
| 关 键 词： | 前脂肪细胞 脂联素基因 激素敏感脂肪酶基因 乳牛 |
| 文章编号： | 1673-4696(2007)06-0510-05 |
| 修稿时间： | 2007-01-10 |
Detection of expression levels of ADPN mRNA and HSL mRNA of dairy cow preadipocytes in different stages |
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| ZHANG Hui,CHANG Wei-yi,ZHANG Cai,SONG Wen-hua,WANG Zhe. Detection of expression levels of ADPN mRNA and HSL mRNA of dairy cow preadipocytes in different stages[J]. Chinese Veterinary Science, 2007, 37(6): 510-514 | |
| Authors: | ZHANG Hui CHANG Wei-yi ZHANG Cai SONG Wen-hua WANG Zhe |
| Abstract: | Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method,and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture,respec-tively.The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.The results showed that the abundance of ADPN mRNA was up-regulated during the differentiation period(on day 2 to 12),but was down-regulated during post-differentiation(on day 14 to 16),and HSL mRNA was decreased on day 10-16 post-cultivation. |
| Keywords: | preadipocyte ADPN gene HSL gene dairy cow |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |