尿中地西泮及其代谢物的气相色谱电子捕获检测法分析

目的　建立尿中地西泮及其代谢物替马西泮、去甲西泮和奥沙西泮的GC/ECD分析方法。方法　尿样经β 葡萄糖醛酸酶水解后 ,用有机溶剂提取 ,再以N ,O 双三甲硅烷基三氟乙酰胺进行衍生化 ,衍生物用GC/ECD法分析。结果　检材中分析物的回收率 70 %以上 ,检出限 5ng/ml以下。结论　本法可进行口服地西泮 10mg人体 48h内尿液中地西泮代谢物的分析。

Analysis of diazepam and its main metabolites in urine by GC-ECD

Objective A method was developed for the determination diazepam and its main metabolites,nordiazepam, and oxazepam and then in urine by GC-ECD. Method The urine samples were hydrolyzed with β-glucuronidase were extracted in organic solvent. The extractives were derived with BSTFA and the analytes as trimethylsilyl derivatives were determined by GC. Results Sensitive of the method is as low as snglme, the recovery is high then 70%. Conclusion This method is sensitive enough for the analysis of urine from the subjects over a 48 hour period after receiving a 10mg dose of diazepam orally.