The use of isoelectric focusing (IEF) as a method for the combined phenotyping of erythrocyte acid phosphatase (EAP) and esterase D (EsD) |
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Authors: | S T Finney N A Renshaw D J Werrett |
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Institution: | Home Office Forensic Science Laboratory, Priory House, Gooch St. North Birmingham B5 6QQ U.K. |
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Abstract: | The simultaneous isoelectric focusing (IEF) in polyacrylamide gels (PAG) of erythrocyte acid phosphatase (EAP) and esterase D (EsD) allows the poor discriminating power (DP) of EsD to be usefully combined with a highly discriminating system EAP, such that a joint DP of 0.766 was achieved compared with PGM IEF DP 0.756. Focusing was carried out in a centrally flattened gradient containing ampholines (pH 4-6 and 6-8) and the chemical spacer 3-(N-morpholino) propanesulphinic acid (MOPS). It enabled the identification of six EsD phenotypes including the recently discovered EsD5 isozymes. The application of this method to casework bloodstains is discussed. |
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Keywords: | Isoelectric focusing Combined phenotyping Erythrocyte acid phosphatase Esterase D |
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