Enantiomeric separation of amphetamine by high-performance liquid chromatography using chiral crown ether-coated reversed-phase packing: application to forensic analysis |
| |
| Affiliation: | 1. CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317 Gandra, PRD, Portugal;2. Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, Portugal;3. Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, s/n, Matosinhos, Portugal;4. LAQV-Requimte, Laboratório de Bromatologia e Hidrologia, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, Portugal;5. ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal |
| |
| Abstract: | d-Amphetamine and l-amphetamine were clearly separated by HPLC analysis using a chiral crown ether column (CROWNPACK CR(+)). As little as 0.1% d-amphetamine in bulk methamphetamine could be determined. The enantiomeric form of the by-product in a drug sample may be helpful in evaluating the possibility of illicit synthesis. When the isomeric composition of amphetamine present in urine from a methamphetamine abuser was examined, only d-amphetamine was detected as a metabolite. The present method was also applied to the enantiomeric separation of norephedrine. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
