大鼠尺神经损伤平面和修复时间对爪内肌细胞凋亡的影响

目的研究尺神经损伤修复后靶肌功能恢复的影响因素。方法42只SD雄性大鼠分为肘部、腕部2组实验组和1组正常对照组；实验组在肘部和腕部完全离断两前肢尺神经后即刻、1个月、3个月修复一侧神经（另一侧为对照侧），应用免疫组织化学染色和电镜技术，观察和比较大鼠爪内肌细胞凋亡及细胞超微结构变化。结果即刻修复组大鼠爪内肌促细胞凋亡基因Fas、Caspase-3表达与正常组之间的差异无统计学意义；随修复时间延长，其表达逐渐增高，且组间有显著性差异（P〈0．05）。抑细胞凋亡基因Bcl-2蛋白表达随修复时间延长而逐渐降低。伤后即刻、1月组实验侧Fas和Caspase-3蛋白表达较相应对照侧低（P〈0．05）；而3月组实验侧与相应对照侧比较无明显差异（P〉0．05）；相同修复时间，不同损伤平面组间表达差异均无统计学意义。结论靶肌超微结构改变及细胞凋亡的发生与神经损伤后修复时间有密切关系，与损伤平面关系较小。在法医学鉴定时机认定方面，尚需进一步研究验证。

Apoptosis and uitrastructure changes in claw muscles of rats at different injury level and restoration time of ulnar nerve

Objective To study the factors influencing the ultrastructure and apoptosis frequency on target muscle when the ulnar nerve was cut off by different injury level(IL) and repaired at different restoration time(RT).Methods 42 SD rats were randomly divided into 3 groups,including 2 experimental groups with IL at wrist and elbow separately and 1 normal control group.The ulnar nerves of two front limbs were cut at elbow level or wrist level and the nerve of one side was repaired at different time (immediately,1 ...