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| 05式警用转轮手枪弹壳接触DNA检验方法的比较 | |
| 引用本文： | 郭磊,周茹华,白雪,赵蕾,郑其双,姜伯玮,赵兴春,叶健. 05式警用转轮手枪弹壳接触DNA检验方法的比较[J]. 中国法医学杂志, 2013, 28(3): 230-232,265 |
| 作者姓名： | 郭磊 周茹华 白雪 赵蕾 郑其双 姜伯玮 赵兴春 叶健 |
| 作者单位： | 1. 中国人民公安大学,北京,100038 2. 苏州市公安局,江苏苏州,215000 3. 公安部物证鉴定中心,北京,100038 4. 中科院上海应用物理所,上海,201800 |
| 摘 要： | 目的比较05式警用转轮手枪弹壳表面接触性DNA检验方法,为实际检验提供参考和借鉴。方法制备40例击发后手枪弹壳的模拟样本,分别用两步转移法提取弹壳表面不同部位检材,采用两种DNA提取法和两种扩增试剂盒对样本进行STR分型检验,比较评价检验结果。结果避开发射药残留区域采用两步转移法提取样本,有助于提高检出率;Chelex-100联合Microcon-100法提取模板DNA的产量最高可达1.18ng,高于Mini M48试剂盒法(0.91ng);MiniFilerTM试剂盒的等位基因检出率(23.61%)高于IdentifilerTM试剂盒(6.41%)。结论采用选择适当区域提取检材,采用Chelex-100联合Microcon-100法提取DNA,经MiniFilerTM试剂盒扩增,进行弹壳接触DNA分型的效果较好。 |
| 关 键 词： | 法医物证学 枪弹壳 接触DNA 检验方法 STR |
DNA typing methods comparison of Typer 2005 police 9 mm revolver cartridge |
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| Guo Lei,Zhou Ruhua,Bai Xue,Zhao Lei,Zheng Qishuang,Jiang Bowei,Zhao Xingchun,Ye Jian. DNA typing methods comparison of Typer 2005 police 9 mm revolver cartridge[J]. Chinese Journal of Forensic Medicine, 2013, 28(3): 230-232,265 | |
| Authors: | Guo Lei Zhou Ruhua Bai Xue Zhao Lei Zheng Qishuang Jiang Bowei Zhao Xingchun Ye Jian |
| Affiliation: | 1.China People’s Public Security University,Beijing 100038,China;2.Suzhou Municipal Public Security University,Jiangsu Suzhou 215000,China;3.Institute of Forensic Science Ministry of Public Security,Beijing 100038,China;4.Shanghai Insititute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China) |
| Abstract: | Objective Comparing DNA typing methods of fired Typer 2005 police 9mm revolver cartridge,to provide reference for DNA testing in actual shooting cases.Methods 40 mock samples of fired catridge were prepared;Alleles prevalence in different sample transfer parts and the DNA quantification results of different DNA extraction method were compared,also STR typing results of IdentifilerTM and MiniFilerTM kit were compared.Results DNA yield of chelex method combined Microcon-100 column is higher than method of Mini M48 kit method;Avoiding the part of firearms residue could improve alleles prevalence;the allele prevalence of MiniFilerTM kit is 23.61%,which was higher than the allele prevalence of IdentifilerTM kit,6.41%.Conclusion Choosing chelex method combined Microcon-100 column,STR typing results of MiniFilerTM kit are better. |
| Keywords: | forensic biological evidence fired catridge touch DNA test method STR |
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