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| 原发性脑干损伤NF表达定量研究 | |
| 引用本文： | 姚青松,闵建雄,郎建英,宋一璇. 原发性脑干损伤NF表达定量研究[J]. 中国法医学杂志, 2009, 24(5): 301-303 |
| 作者姓名： | 姚青松 闵建雄 郎建英 宋一璇 |
| 作者单位： | 1. 广州市刑事科学技术研究所,广东,广州,510030 2. 公安部物证鉴定中心,北京,100038 3. 广州中医药大学统计学教研室,广东,广州,510405 |
| 基金项目： | 公安部科技基金资助项目 |
| 摘 要： | 目的探讨脑干神经丝蛋白的定量分析对短时间内死亡的原发性脑干损伤鉴定的法医学意义。方法14例原发性脑干损伤者的脑干标本（损伤组）和15例脑外疾病死亡者的脑干标本（对照组）,分别取中脑、脑桥及延脑组织,作NF广谱免疫组化染色,用OPTIMAS 6.0系统图像分析仪定量检测脑干的神经丝蛋白表达,所得数据进行统计学对比处理。结果中脑、脑桥、延脑的NF阳性表达率分别为75.84±23.62（对照组45.17±17.95）,65.94±14.61（对照组42.99±24.12）和83.30±26.66（对照组53.50±28.14）,损伤组与对照组的NF阳性物含量在中脑、脑桥及延脑等3个部位中均有显著差异,P〈0.001或〈0.01。结论定量分析人脑干神经丝蛋白的含量变化,超出常人数量对鉴定早期原发性脑干损伤有价值。 |
| 关 键 词： | 法医病理学 原发性脑干损伤 神经轴索 神经丝蛋白 免疫组化 |
Semi-quantitative analysis of NF expression in primary brainstem injury by image analysis system |
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| YAO Qingsong,MIN Jianxiong,LANG Jianying,SONG Yixuai. Semi-quantitative analysis of NF expression in primary brainstem injury by image analysis system[J]. Chinese Journal of Forensic Medicine, 2009, 24(5): 301-303 | |
| Authors: | YAO Qingsong MIN Jianxiong LANG Jianying SONG Yixuai |
| Affiliation: | (YAO Qingsong1,MIN Jianxiong2,LANG Jianying3,SONG Yixuai1/1.Criminal &Technology Institute,Guangzhou 510030,China,2.Institute of Forensic Science Ministry of Public Security,Beijing 100038,3.Guangzhou University of Chinese Medicine,Guangzhou 510405,China) |
| Abstract: | Objective To investigate the diagnositic value of NF expression in primary brain stem injury by image analysis.Methods Brainstem tissue samples were taken from a total of 29 cases,including 14 cases of primary brainstem injury(injury group) and 15 cases of non-brain stem injury(control group) for image analysis of NF expression detected by immunohistochemical staining.In each case,three regions were selected form midbrain,pons and medulla respectively.Semi-quantitative analysis of NF expression was performe... |
| Keywords: | forensic pathology primary brainstem injury axonal NF immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |