白细胞介素-18在早期心肌缺血和心肌梗死心肌的表达

目的探讨白细胞介素-18(IL-18)对早期心肌缺血(EMI)和心肌梗塞(MI)死后诊断的法医学意义。方法筛选出34例心脏标本,分EMI(11例)、MI(10例)和对照(13例)组,检测心肌IL-18免疫组化染色(SP法)反应,并经全自动图像分析系统分析得到平均光密度值(AOD),所得数据进行统计学分析。结果对照组心肌IL-18免疫组化染色呈阴性,EMI组心肌呈阳性,MI组心肌呈强阳性;其AOD值分别是0.1193±0.0649、0.3827±0.0868、0.4792±0.0884,3组之间差异均具有显著性意义(P<0.05)。结论作为判断EMI和MI的指标之一,IL-18对EMI和MI的死后诊断具有法医学意义。

An immunohistochemical study on IL-18 in early myocardial ischemia and myocardial infarction

Objective To investigate applicability of interleukin-18(IL-18) to diagnosing of early myocardial ischemia(EMI) and myocardial infarction(MI) in postmortem specimens.Methods Myocardial samples collected from 34 atuopsied cases were employed in the experiment,which were divided into 3 groups,EMI(11 cases),MI(10 cases),and control(13 cases).Immunoreactivity of IL-18 detected by immunohistochemical staining(S-P method),and average optical density(AOD) were analyzed by automatic image analysis technique.The data were processed statistically.Results Weak or negative immunoreactivity of IL-18 was noted in the normal myocardial spcimens(NM),positive in the EMI and significantly positive in the MI spcimens.AOD of immunostaining was measured as 0.1193±0.0649,0.3827±0.0868 and 0.4792±0.0884,respectively.There were significant difference between two among the three groups(P<0.05).Conclusion It is indicated that IL-18 can be served as an predictor of EMI and MI,which may provide an method for postmortem diagnosis of EMI and MI.