两种方法提取汗潜手印DNA的比较研究 |
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引用本文: | 郝金萍,邵丽芳,陈松,刘开会,郭燕霞. 两种方法提取汗潜手印DNA的比较研究[J]. 刑事技术, 2013, 0(4): 34-35 |
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作者姓名: | 郝金萍 邵丽芳 陈松 刘开会 郭燕霞 |
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作者单位: | 1. 公安部物证鉴定中心,北京,100038 2. 北京市公安局刑侦总队七支队,北京,100054 |
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摘 要: | 目的比较M48和DNeasy○R plant Mini两种方法提取汗潜手印DNA的优劣。方法用M48和DNeasy○Rplant Mini两种方法分别提取16对汗潜手印DNA,并进行DNA定量,比较定量结果。结果 M48法明显比plant Mini法提取到的DNA量多(配对t检验:α=0.05,t=3.45,γ=15,0.002
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关 键 词: | 法庭科学 汗潜手印 DNA提取 |
The comparison of two methods for extracting DNA from sweat latent fingerprints |
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Affiliation: | HAO Jin-ping,SHAO Li-fang,CHEN Song,et al(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
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Abstract: | Objective To compare QIAGEN MagAttract○ R DNA Mini M48 kit and DNeasy○ R plant Mini kit in DNA extraction from sweat fingerprints.Methods DNA on 16 pairs of fingerprints was extracted using QIAGEN MagAttract○ R DNA Mini M48 kit and DNeasy○ R plant Mini kit respectively,and then the extract was analyzed.Results A significant increase in DNA recovery was noted by using QIAGEN MagAttract○ R DNA Mini M48 kit as compared to DNeasy○ R plant Mini kit(pared t-test:α=0.05,t=3.45,γ=15,0.002p0.005).Conclusion The QIAGEN MagAttract○ R DNA Mini M48 kit was more effective for DNA recovery from sweat latent fingerprints compared with the DNeasy○ R plant Mini kit. |
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Keywords: | forensic science sweat latent fingerprints DNA extraction |
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