| 红细胞酯酶D(EsD)型的分布及血痕EsD型的检出 |
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| 引用本文： | 王志贤,贾静涛,王秀玲. 红细胞酯酶D(EsD)型的分布及血痕EsD型的检出[J]. 中国法医学杂志, 1986, 0(2) |
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| 作者姓名： | 王志贤 贾静涛 王秀玲 |
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| 作者单位： | 中国医科大学法医学系法医血清学教研室，中国医科大学法医学系法医血清学教研室，中国医科大学法医学系法医血清学教研室 |
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| 摘 要： | 本文应用高压琼脂糖凝胶电泳法对沈阳地区汉族221例随机献血者的红细胞酯酶D(EsD)型进行了检测,其基因频率为 EsD~1=0.629,EsD~2=0.371。结合文献资料分析了EsD 型分布的种族差异,发现黄种人的 EsD~1频率最低,白种人及黑人最高。用本法对红细胞溶血液的最小检出量为1μl;以1μl 溶血液作成的血痕检样,室温保存2周仍可分型。以5μl 溶血液作成的血痕检样可分型时间为3周;37℃保存的溶血液可分型时间为7～8天。
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| 关 键 词： | 法医血清学 红细胞酯酶 D 型 基因频率 血痕 |
INVESTIGATION ON THE DISTRIBUTION OF ESTERASE D(EsD)PHENOTYPES AND THE DETECTION OF EsD FROM BLOODSTAINS |
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| Wang Zhixian Jia Jingtao Wang Xiuling. INVESTIGATION ON THE DISTRIBUTION OF ESTERASE D(EsD)PHENOTYPES AND THE DETECTION OF EsD FROM BLOODSTAINS[J]. Chinese Journal of Forensic Medicine, 1986, 0(2) |
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| Authors: | Wang Zhixian Jia Jingtao Wang Xiuling |
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| Affiliation: | Wang Zhixian Jia Jingtao Wang Xiuling Department of Forensic Serology,Faculty of Forensic Medicine,China Medical University |
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| Abstract: | The phenotyping of erythrocyte esterase D of 221 Chinese random donors,Han nationalities in Shenyang,China,was performed by the agarose gel electrophoresis.Gene frequencies were:E_SD~1=0.629,E_SD~2= 0.371.There is no significant difference of gene frequencies of Han nationalities between Shenyang and Beijing.The minimal amount meeted the requirment for detection of E_S D were 1μl of fresh hemolysate or 1μl hemolysate.The E_S D can be detected from the hemolysates kept in 37℃ for 7～8days as well as from 1μl hemolysate bloodstain kept for 2 weeks and 5μl hemolysate bloodstain kept for 3 weeks. |
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| Keywords: | Forensic serology Es D phenotypes Gene frequency Bloodstains |
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