| PR8流感病毒突变株的制备及其在鸡胚上生长特性的研究 |
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| 引用本文： | 王朝霞,滕巧泱,戴晓光,黄海碧,张树梅,张旭,徐大伟,李培锋,李泽君. PR8流感病毒突变株的制备及其在鸡胚上生长特性的研究[J]. 中国兽医科学, 2010, 40(8) |
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| 作者姓名： | 王朝霞 滕巧泱 戴晓光 黄海碧 张树梅 张旭 徐大伟 李培锋 李泽君 |
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| 作者单位： | 王朝霞(内蒙古农业大学,兽医学院,内蒙古,呼和浩特,010018;中国农业科学院,上海兽医研究所,农业部寄生虫学重点开放实验室,上海,200241);滕巧泱,戴晓光,黄海碧,张树梅,张旭,徐大伟,李泽君(中国农业科学院,上海兽医研究所,农业部寄生虫学重点开放实验室,上海,200241);李培锋(内蒙古农业大学,兽医学院,内蒙古,呼和浩特,010018) |
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| 基金项目： | 上海市浦江人才计划项目，转基因生物新品种培育重大专项 |
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| 摘 要： | 在前期发现流感病毒WSN株的NS2或NP蛋白突变使病毒在细胞上增殖能力明显提高的基础上,研究了这些突变是否会使PR8病毒效价在鸡胚上进一步提高的可行性,通过反向遗传操作技术获得了NS2E67S和NPP277A两株PR8突变病毒。经过比较突变病毒与野生病毒在鸡胚中的增殖能力,发现NS2E67S突变病毒在鸡胚上增殖后的效价与HA抗原表达量均明显提高,而NPP277A突变病毒在鸡胚上的增殖能力不及野生PR8病毒。该研究结果将对进一步研究病毒基因变异与宿主互作机制奠定基础,有可能通过NS2E67S突变病毒对改良流感疫苗具有重要的应用价值。
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| 关 键 词： | 流感病毒 突变株 |
Development of influenza virus PR8 mutants and their characteristics on embryonated chicken eggs |
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| WANG Zhao-xia,,TENG Qiao-yang,DAI Xiao-guang,HUANG Hai-bi,ZHANG Shu-mei,ZHANG Xu,XU Da-wei,LI Pei-feng,LI Ze-jun. Development of influenza virus PR8 mutants and their characteristics on embryonated chicken eggs[J]. Chinese Veterinary Science, 2010, 40(8) |
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| Authors: | WANG Zhao-xia TENG Qiao-yang DAI Xiao-guang HUANG Hai-bi ZHANG Shu-mei ZHANG Xu XU Da-wei LI Pei-feng LI Ze-jun |
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| Affiliation: | WANG Zhao-xia1,2,TENG Qiao-yang2,DAI Xiao-guang2,HUANG Hai-bi2,ZHANG Shu-mei2,ZHANG Xu2,XU Da-wei2,LI Pei-feng1,LI Ze-jun2(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China,2.Key Laboratory of Animal Parasitology of the Ministry of Agriculture/Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China) |
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| Abstract: | In previous studies,we found that some A/WS/33(WSN) mutants on NS2 and NP proteins showed increasing replication abilities on MDCK cells.In order to characterize the amplification of PR8 mutants on these proteins in embryonated chicken eggs,the mutants were obtained by PCR-mutation and influenza virus reverse genetic technology.After being replicated in chicken embryonated eggs,the viral titer and HA titer of mutant NS2 E67S were higher than that of wt PR8,while these titers of NP P277A were lower than that... |
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| Keywords: | PR8 |
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