A new individualization marker of semen: deoxyribonuclease I (DNase I) polymorphism. |
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Authors: | K Sawazaki T Yasuda D Nadano E Tenjo R Iida H Takeshita K Kishi |
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Affiliation: | Department of Legal Medicine, Fukui Medical School, Japan. |
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Abstract: | We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen. |
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