用多种探针药物评价穿心莲对鸡药物代谢酶活性的影响

采用咖啡因、氨苯砜、氯唑沙宗、奥美拉唑药物探针法,测定这些探针药物在鸡体内的消除半衰期,以探讨穿心莲超微粉对鸡的药物代谢酶的影响,结果显示,对照组和试验组的咖啡因t1/2β分别为632min、415 min,表明两组的t1/2β之间有显著差异(P<0.05).对照组与试验组的氨苯砜t1/2β分别为55.4 min、48.4 min,二者之间差异不显著(P>0.05).对照组和试验组的奥美拉唑t1/2β分别为16.5 min、7.6 min,表明两组的消除半衰期差异极显著(P<0.01);氯唑沙宗t1/2β分别为26.3 min、20.5 min,两组差异显著(P<0.05).结果表明,穿心莲超微粉对CYP3A4、CYP1A2、CYP2E1和CYP2C19的活性都有诱导作用,但与对照组相比,对CYP3A4的诱导作用差异不显著,而对CYP2C19的诱导作用差异极显著.

Evaluation of influence of Andrographis paniculata on activities of CYP1A2,CYP3A4,CYP2E1 and CYP2C19 genotypes in chickens by multiple cytochrome P450 probe substrates

To study the effects of Andrographis paniculata ultra-fine powder on drug metabolic en-zymes in chickens,the four drug probes,caffeine,aminophenylsulfone,omeprazole and chlorzoxazone in vi-vo , were used in the tests,and the elimination half time of the approaches was determined. The t_(1/2β) in the control group and test group of caffeine was 632 min and 415 min respectively,and there was significantly difference between them(P<0. 05). The counterparts of aminophenylsulf one, omeprazole and chlorzoxa-zone were 55.4 and 48.4 min(P>0. 05),16. 5 and 7. 6 min(P<0. 01),26. 3 and 20. 5 min(P<0. 05), re-spectively. The conclusions were that Andrographis paniculata ultra-fine powder could induce the meta-bolic enzyme activities of CYP1A2, CYP2E1 and CYP2C19 significantly, CYP3A4 not significantly, CYP2C19 in strongly-marked differences.