| 建立检测制式长枪上DNA多态性的新方法 |
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| 引用本文： | 杨秀乔,苗琳,谢洪良,黄磊,苏世达,潘顺勇,曾发明,翟滇,程宝文. 建立检测制式长枪上DNA多态性的新方法[J]. 中国法医学杂志, 2017, 0(4). DOI: 10.13618/j.issn.1001-5728.2017.04.013 |
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| 作者姓名： | 杨秀乔 苗琳 谢洪良 黄磊 苏世达 潘顺勇 曾发明 翟滇 程宝文 |
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| 作者单位： | 1. 昆明医科大学法医学院,云南 昆明,650500;2. 玉溪市公安局,云南 玉溪,653100;3. 曲靖市公安局,云南 曲靖,655000;4. 昆明市公安局,云南 昆明,650034;5. 迪庆州公安局,云南 迪庆,674400;6. 云南省公安厅刑事科学技术研究所,云南 昆明,650500 |
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| 基金项目： | 云南省科技惠民计划(2013CA013) |
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| 摘 要： | 目的建立快速、准确检测制式长枪上基因分型的新方法。方法联合使用直扩法、硅膜法对48支制式长枪上5个不同部位共240份检材进行DNA检测。结果联合使用直扩法及硅膜法,在48支制式长枪中检测出DNA完整分型42支,检出率达87.50%。结论联合使用直扩法、硅膜法,可有效快速、准确地检测出制式长枪上DNA多态性。
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| 关 键 词： | 法医物证学 直扩法 硅膜法 制式长枪 基因分型 |
A new methodology of DNA polymorphism detection from standard long gun |
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| Yang Xiuqiao,Miao Lin,Xie Hongliang,Huang Lei,Su Shida,Pan Shunyong,Zeng Faming,Zhai Dian,Cheng Baowen. A new methodology of DNA polymorphism detection from standard long gun[J]. Chinese Journal of Forensic Medicine, 2017, 0(4). DOI: 10.13618/j.issn.1001-5728.2017.04.013 |
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| Authors: | Yang Xiuqiao Miao Lin Xie Hongliang Huang Lei Su Shida Pan Shunyong Zeng Faming Zhai Dian Cheng Baowen |
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| Abstract: | Objective A new methodology was established to efficiently obtain the genotype of DNA remained on standard long gun. Methods Direct PCR and silicon membrane method were combined to detect DNA polymorphism of a total of 240 samples at 5 different positions from 48 standard long guns. Results Combining direct PCR and silicon membrane method, we obtained full DNA profiles in 42 out of 48 standard long guns, with a detection rate up to 87.50%. Conclusion The results demonstrate that the combination of direct PCR and silicon membrane method provide a quick and accurate way to detect DNA polymorphism on the standard long gun. |
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| Keywords: | forensic biological evidence direct PCR silicon membrane method standard long gun genotype |
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