| DART-MS/MS快速检测人血中的3种合成大麻素 |
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| 引用本文： | 闵涛,张瑛,刘少丹,张大明. DART-MS/MS快速检测人血中的3种合成大麻素[J]. 中国法医学杂志, 2017, 0(4): 388-392. DOI: 10.13618/j.issn.1001-5728.2017.04.014 |
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| 作者姓名： | 闵涛 张瑛 刘少丹 张大明 |
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| 作者单位： | 1. 山西医科大学法医学院,山西 太原,030001;2. 山西医科大学法医学院,山西 太原 030001;北京市公安司法鉴定中心法庭毒物分析公安部重点实验室,北京 100192;3. 浙江迪安鉴定科学研究院,浙江 杭州,310000;4. 北京市公安司法鉴定中心法庭毒物分析公安部重点实验室,北京,100192 |
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| 基金项目： | 公安部科技强警基础工作专项(2014GABJC002) |
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| 摘 要： | 目的使用实时直接分析-串联质谱,建立快速检测人血中的JWH-018、JWH-250和AM-2201的方法。方法用乙腈-甲醇(4:1)沉淀蛋白的方法对血样进行简单前处理,采用DART 12Dip-it自动进样系统,以正离子、MRM模式进行分析。结果血液中JWH-018、JWH-250、AM-2201可以得到有效检测,在0.02-5.00μg/m L线性关系良好,相关系数均大于0.99,检出限分别为0.016μg/m L,0.003μg/m L和0.017μg/m L,日内、日间RSD均小于15%。结论本文所建方法灵敏度高,准确性较好,方法省时省力,可用于实际案例血液中合成大麻素JWH-018、JWH-250、AM-2201的分析。
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| 关 键 词： | 法医毒物分析 实时直接分析质谱 合成大麻素 快速检测 |
Rapid screening of 3 synthetic cannabinoids in blood by direct analysis in real time mass spectrometry |
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| Min Tao,Zhang Ying,Liu Shaodan,Zhang Daming. Rapid screening of 3 synthetic cannabinoids in blood by direct analysis in real time mass spectrometry[J]. Chinese Journal of Forensic Medicine, 2017, 0(4): 388-392. DOI: 10.13618/j.issn.1001-5728.2017.04.014 |
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| Authors: | Min Tao Zhang Ying Liu Shaodan Zhang Daming |
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| Abstract: | Objective To establish a method for rapid detection of JWH-018, JWH-250 and AM-2201 in blood by direct analysis in real-time coupled with tandem mass spectrometry. Methods These samples were extracted by acetonitrile-methanol (4:1), using DART 12Dip-it automatic sampling system. They were analyzed by positive ion and MRM mode. Results The detection limits of JWH-018, JWH-250 and AM-2201 were in good linearity in the range of 0.02-5.00μg/mL. The correlation coefficients were above 0.99 and the detection limits were 0.016μg/mL, 0.003μg/ mL and 0.017μg/mL, respectively. The intra-day and inter-day RSD were less than 15%. Conclusion The method has the advantages of high sensitivity and good accuracy. The sample processing is simple and can be analyzed in short time. This method is suitable for the analysis of JWH-018, JWH-250 and AM-2201 in practical cases. |
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| Keywords: | forensic toxicological analysis direct analysis in real-time mass spectrometry synthetic cannabinoids rapid screening |
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