| 固相萃取-液相色谱-质谱法检验人全血中的地塞米松 |
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| 引用本文： | 栾玉静,王瑞花,董颖,张蕾萍,于忠山. 固相萃取-液相色谱-质谱法检验人全血中的地塞米松[J]. 中国法医学杂志, 2017, 0(3): 297-299. DOI: 10.13618/j.issn.1001-5728.2017.03.017 |
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| 作者姓名： | 栾玉静 王瑞花 董颖 张蕾萍 于忠山 |
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| 作者单位： | 公安部物证鉴定中心 北京市现场物证检验工程技术研究中心,北京,100038 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金计划项目(2013JB009) |
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| 摘 要： | 目的采用固相萃取结合液相色谱-质谱法检验人全血中地塞米松。方法采用SPE提取全血,用UPLC-MS/MS方法测定,采用ESI离子源,MRM方式监测。结果地塞米松的检出限为0.05ng/mL,线性范围1ng/mL~100ng/mL,方法回收率大于78.1%,日内、日间精密度均小于15%。结论 SPE-UPLC/MS/MS检验人全血中地塞米松的方法简单、高效,可应用于人全血中地塞米松的检验。
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| 关 键 词： | 法医毒物分析 固相萃取 超高效液相色谱-质谱 地塞米松 |
Determination of dexamethasone in human plasma by solid phase extraction with ultra performance liquid chromatography-tandem mass spectrometer |
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| Luan yujing,Wang ruihua,Dong ying,Zhang Leiping,Yu zhongshan. Determination of dexamethasone in human plasma by solid phase extraction with ultra performance liquid chromatography-tandem mass spectrometer[J]. Chinese Journal of Forensic Medicine, 2017, 0(3): 297-299. DOI: 10.13618/j.issn.1001-5728.2017.03.017 |
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| Authors: | Luan yujing Wang ruihua Dong ying Zhang Leiping Yu zhongshan |
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| Abstract: | Objective To develop the analytical method to determine the content of dexamethasone in human plasma by solid phase extraction with ultra performance liquid chromatography-tandem mass spectrometer. Methods The human plasma was extracted with a solid phase extraction(SPE) and determined by UPLC-MS/MS. LC-MS/MS was performed in ESI source with MRM mode for quantification. Results The lowest detectable limit was 0.05ng/mL, the linear range was 1~100ng/mL. The absolute recovery was more than 78.1%. The intra- and inter day precision was within 15% at three concentrations. Conclusion Since the procedure proved to be simple, quick and effective, it could be used for the determination of dexamethasone in human plasma. |
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| Keywords: | forensic toxicological analysis solid phase extraction UPLC-MS/MS dexamethasone |
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