| 开发高灵敏度血痕发光试剂用于法医现场检测 |
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| 引用本文： | 史月群,刘未斌,秦瑛,张丽艳,李震. 开发高灵敏度血痕发光试剂用于法医现场检测[J]. 中国法医学杂志, 2017, 0(2): 179-181. DOI: 10.13618/j.issn.1001-5728.2017.02.016 |
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| 作者姓名： | 史月群 刘未斌 秦瑛 张丽艳 李震 |
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| 作者单位： | 1. 广东省珠海市公安司法鉴定中心,广东珠海,519075;2. 广州九贤林生物科技有限公司,广东广州,510000 |
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| 基金项目： | 公安部应用创新计划项目(2015YYCXGDST024) |
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| 摘 要： | 目的开发高灵敏度血痕发光试剂用于法医现场检测。方法本项目通过借鉴ECL发光的原理,通过化学电子链的乒乓共轭激活原理,使用均匀设计实验方法优化条件,合成多个新化合物,开发出新型高灵敏度血痕发光试剂。结果本项目开发的高灵敏度血痕发光试剂灵敏度高,发光时间长,在血液稀释1000倍的情况下,使用Chemi Scope3300化学发光成像系统读取荧光值,经过灰度转化最高值达到56,发光时间达到10分钟。结论本项目成功开发了高灵敏度血痕发光试剂,可以应用于法医现场检测。
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| 关 键 词： | 法医物证学 血痕 化学发光 |
The application of a high-performance bloodstain testing reagent in forensic scene |
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| Shi Yuequn,Liu Weibin,Qin Ying,Zhang Liyan,Li Zhen. The application of a high-performance bloodstain testing reagent in forensic scene[J]. Chinese Journal of Forensic Medicine, 2017, 0(2): 179-181. DOI: 10.13618/j.issn.1001-5728.2017.02.016 |
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| Authors: | Shi Yuequn Liu Weibin Qin Ying Zhang Liyan Li Zhen |
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| Abstract: | Objective To develop a highly sensitive luminescent reagent for bloodstain testing at forensic crime scenes.Methods Based ontheprincipleof ECL luminescence and the ping-pong conjugate activation principle of chemical electronic chain,this project developed a new type of highly sensitive luminescent reagent for bloodstain testing by usingthe uniform design of experimental methods to optimize the conditions andsynthesize several new compounds.Results The bloodstain testing luminescent reagentdeveloped in this project has high sensitivity andlongluminescence time.In the case of blood samples diluted by 1,000 times,reading the fluorescence withChemiScope 3300 chemiluminescence imaging system,the maximumvalue of gray scale reached 56,and the luminescence time lasted for 10 minutes.Conclusion The project has successfully developed a highly semitivebloodstain testing reagentthat could be applied to crime scene investigation. |
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| Keywords: | forensic biological evidence bloodstain chemiluminescence |
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