| 染色混合精斑涂片DNA检验方法的研究 |
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| 引用本文： | 吴微微,丁宏,应宗敏,徐林苗,郑秀芬. 染色混合精斑涂片DNA检验方法的研究[J]. 刑事技术, 1999, 0(6) |
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| 作者姓名： | 吴微微 丁宏 应宗敏 徐林苗 郑秀芬 |
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| 作者单位： | 浙江省公安厅刑警总队 杭州310009(吴微微，丁宏，应宗敏，徐林苗)，公安部物证鉴定中心 北京100038(郑秀芬) |
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| 摘 要： | 建立了染色混合精斑涂片DNA的提取方法,通过两步扩增的技术与垂直聚丙烯酰胺凝胶电泳及银染色法,成功检测了3个STR位点.结果显示,所有染色涂片DNA分型均与对照血样一致,苏木素-伊红(HE)染色比酸性复红-美蓝(Baecchi)染色对DNA的降解作用要小;涂片灵敏度检验可达到少于10个精子,且室温保存一年以内的涂片其灵敏度光显著差异.该方法简单、快速、可靠,为染色混合精斑涂片的PCR分析提供了新方法.
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| 关 键 词： | 混合精斑 染色 细胞涂片 两步扩增 短串联重复序列(STR) |
A STUDY ON TESTING OF DNA FROM STAINED SMEARS OF MIXED SEMINAL STAIN |
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| Wu Weiwei,et al.. A STUDY ON TESTING OF DNA FROM STAINED SMEARS OF MIXED SEMINAL STAIN[J]. Forensic Science and Technology, 1999, 0(6) |
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| Authors: | Wu Weiwei et al. |
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| Affiliation: | Wu Weiwei,et al. Department of criminal investigation,Zhejiang provincial public security bureau,Hangzhou 310009 |
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| Abstract: | A method of extraction of DNA from stained smears of mixed seminal and vaginal stain was established by using the two - step amplification, polyacrylamide gel vertical electrophoresis and silver staining techniques. Three STR loci have been performed successfully. The results show that all DNA - typing of the stained smears were consistent with the corresponding blood; the smear slides stained with hematoxylin-eosic(HE) made the samples' DNA less degraded than stained with the acid fuchsin and methylene blue(Baecchi); a minimum of spermatozoa was less than 10 for positive results and the sensitivity of smears slides kept within 1 year at room temperature were no difference. The method is rapidity, simplicity and reliability for testing stained smears of mixed seminal stain. |
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| Keywords: | Mixed seminal stain Stained smears Two - step amplification Short tandem repeat (STR) |
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