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| 一种改良骨骼DNA的提取方法 | |
| 引用本文： | 吴微微,徐林苗,李佑英,余洁. 一种改良骨骼DNA的提取方法[J]. 刑事技术, 2001, 0(6): 12-14 |
| 作者姓名： | 吴微微 徐林苗 李佑英 余洁 |
| 作者单位： | 1. 浙江省公安厅刑侦总队, 2. 杭州市公安局刑侦支队, 3. 嘉兴市公安局刑侦支队, |
| 摘 要： | 采用十六烷基三甲基溴化铵(简称CTAB)及两次纯化浓缩的方法提取骨骼DNA,利用荧光标记复合扩增、自动毛细管电泳与分型的技术,成功检测到14个STR位点的分型.该方法稳定、结果可靠,为严重降解骨骼DNA的提取提供了一种可参用的方法. |
| 关 键 词： | 骨骼 DNA提取CTAB纯化浓缩 PCR |
| 修稿时间： | 2001-10-25 |
AN IMPROVED METHOD FOR EXTRACTING DNA FROM SKELETON |
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| Wu Weiwei,Li Youying,Xu Linmiao,et al.. AN IMPROVED METHOD FOR EXTRACTING DNA FROM SKELETON[J]. Forensic Science and Technology, 2001, 0(6): 12-14 | |
| Authors: | Wu Weiwei Li Youying Xu Linmiao et al. |
| Affiliation: | Wu Weiwei,Li Youying,Xu Linmiao,et al.Department of Criminal Investigation,Zhejiang provincial Public Securit y Bureau,Hangzhou 310009 |
| Abstract: | A method of extraction of DNA from bad ly degraded skeleton was established by using CTAB and two times of purifing and concentration.We examined 23cases using Profiler Plus and Cofiler amplification kit with ABI Prism 310Genetic Analyer.14STR loci have been performed successfully.The results showed that it was a relia ble method,and can be used for extraction of DNA f rom highly degraded skeleton. |
| Keywords: | skeleton extraction of DNA CTAB purifing and concentrationNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |