| 鱼类神经坏死病毒的分离及其部分衣壳蛋白基因的核苷酸序列分析 |
| |
| 引用本文： | 李冬. 鱼类神经坏死病毒的分离及其部分衣壳蛋白基因的核苷酸序列分析[J]. 中国兽医科学, 2005, 35(2): 108-111 |
| |
| 作者姓名： | 李冬 |
| |
| 作者单位： | 1. 中国农业科学院,兰州兽医研究所,甘肃,兰州,730046;雅典农业大学,动物科学学院,希腊,雅典,11855
2. 雅典农业大学,动物科学学院,希腊,雅典,11855 |
| |
| 基金项目： | 国家留学基金管理委员会资助项目(CSC22862022) |
| |
| 摘 要： | 将海水鲈鱼和淡水鲟鱼的病鱼脑组织浸出液分别接种 SSN-1 细胞系,传 2~3 代后,收获细胞分离物;参考鱼类神经坏死病毒的全基因序列设计了 2 对引物,用套式 PCR检测细胞分离物均为阳性,并获得了2 个病毒衣壳蛋白部分基因片段,长度分别为 718 bp(鲈鱼)和 263 bp(鲟鱼)。核苷酸序列分析表明,这2株不同来源的病毒均属于 RGNNV基因型,说明不仅在海水养殖环境下而且在淡水鱼中也有鱼类神经坏死病毒存在。
|
| 关 键 词： | 鱼类神经坏死病毒 衣壳蛋白基因 海水鲈鱼 淡水鲟鱼 |
| 文章编号： | 1000-6419(2005)02-0108-04 |
| 修稿时间： | 2004-10-21 |
Isolation of fish nervous necrosis virus and sequencing of their partial capsid protein gene nucleotides |
| |
| LI Dong,Xylouri-Frangiadaki E. Isolation of fish nervous necrosis virus and sequencing of their partial capsid protein gene nucleotides[J]. Chinese Veterinary Science, 2005, 35(2): 108-111 |
| |
| Authors: | LI Dong Xylouri-Frangiadaki E |
| |
| Abstract: | Two sets of primers were designed according to the whole gene sequence of the referenced fish nervous necrosis virus. SSN-1 cell lines were inoculated by the infectious brains of marine seabass or freshwater sturgen separately. The CPE liquids were detected using RT-PCR and nested-PCR after 2 to 3 passages.Two DNA fragments were obtained and sequenced. One is 718bp(from seabass) in length, and the other is 263bp(from sturgen) in length.The sequence comparison showed that the 2 strains of NNV belongs to RGNNV genotype. |
| |
| Keywords: | fish nervous necrosis virus capsid protein gene marine seabass freshwater sturgen |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
