| 地塞米松对人星形胶质细胞凋亡的影响 |
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| 引用本文： | 董丽儒,杨仕坤,连俊波,霍双杰,罗丹,杨虎,宋旭东,丛斌. 地塞米松对人星形胶质细胞凋亡的影响[J]. 中国法医学杂志, 2021, 0(2): 154-157 |
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| 作者姓名： | 董丽儒 杨仕坤 连俊波 霍双杰 罗丹 杨虎 宋旭东 丛斌 |
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| 作者单位： | 华北理工大学附属医院病理科;河北医科大学法医学院 |
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| 基金项目： | 河北省2018年度医学科学研究重点课题(20180767);国家自然科学基金重点项目(81430047)。 |
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| 摘 要： | 目的 观察地塞米松(Dexamethasone,DEX)对人星形胶质细胞(Human astrocytes,HA)凋亡的影响.方法 (1)培养人星形胶质细胞,免疫荧光法检测糖皮质激素受体(Glucocorticoid recepters,GR)的表达;(2)采用MTT实验检测不同浓度的DEX(10-9M～10-5M)对...
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| 关 键 词： | 星形胶质细胞 凋亡 糖皮质激素 糖皮质激素受体 |
Effects of dexamethasone on human astrocyte apoptosis |
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| Dong Liru,Yang Shikun,Lian Junbo,Huo Shuangjie,Luo Dan,Yang Hu,Song Xudong,Cong Bin. Effects of dexamethasone on human astrocyte apoptosis[J]. Chinese Journal of Forensic Medicine, 2021, 0(2): 154-157 |
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| Authors: | Dong Liru Yang Shikun Lian Junbo Huo Shuangjie Luo Dan Yang Hu Song Xudong Cong Bin |
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| Affiliation: | (Department of Pathology,Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,Hebei,China;Forensic Medicine of Hebei Medical University,Hebei Key Laboratory of Forensic Medicine,Shijiazhuang 050017,Hebei,China) |
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| Abstract: | Objective To observe the apoptosis of human astrocytes(HA) by dexamethasone(DEX). Methods(1)Immumofluorescence method was used to examine the expression of GR in human astrocytes.(2) The effects of different concentrations of DEX(10-9 M~10-5 M) on astrocytes survival rate were determined by MTT.(3) Annexin V and PI staining was used to measure the apoptotic rates of astrocyte. Results(1) GR was localized dominantly in the cytoplasm in HA.(2)MTT results indicated that DEX(10-9 M~10-5 M) treatment gradually decreased cell viability compared with control group. 3. Compared with Control group, the apoptotic rate of HA was significantly increased after DEX treatment for 6 h, but the apoptotic rate of HA was decreased after co-treated with RU486. However, there is no significant difference between the Control group and RU486 group. Summary DEX can induce apoptosis of astrocytes independtely. |
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| Keywords: | Astrocyte Apoptosis Glucocorticoid Glucocorticoid recepter |
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