| 线粒体DNA nt8584、nt8701单核苷酸多态性研究 |
| |
| 引用本文： | 郝金萍,韩志义,陈松,李万水,刘开会,郭燕霞,周毅. 线粒体DNA nt8584、nt8701单核苷酸多态性研究[J]. 刑事技术, 2010, 0(6): 19-21 |
| |
| 作者姓名： | 郝金萍 韩志义 陈松 李万水 刘开会 郭燕霞 周毅 |
| |
| 作者单位： | [1]公安部物证鉴定中心法医遗传学公安部重点实验室,北京100038 [2]航空中心医院,北京100012 |
| |
| 摘 要： | 目的建立一种快速、准确的线粒体DNA单核苷酸多态性位点检测方法。方法收集62份无关个体血液样本,应用荧光定量PCR技术研究线粒体DNA nt8584、nt8701位点在中国汉族人群中的多态性。结果 nt8584位点检测到10例nt8584A、52例nt8584G,nt8584A/G变异频率为16∶84;nt8701位点检测到27例nt8701A、35例nt8701G,nt8701A/G变异频率为44∶56。结论所建立的荧光定量PCR方法分型准确、耗时短,适用于法医DNA检验。
|
| 关 键 词： | 线粒体DNA 单核苷酸多态性 荧光定量PCR |
Polymorphism of mtDNA nt8584 and nt8701 based on quantitative real-time PCR |
| |
| Affiliation: | HAO Jin-ping,HAN Zhi-yi,CHEN Song,et al.(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
| |
| Abstract: | Objective To establish a method for mtDNA nt8584 and nt8701 loci polymorphism typing.Methods Using quantitative real-time PCR,the polymorphism of nt8584 and nt8701 loci were detected among 62 unrelated individuals.Results Ten nt8584A and fifty-two nt8584G have been detected in nt8584 loci,whose mutation frequency was 16∶84.Twenty-seven 27 nt8701A and thirty-five nt8701G have been detected in nt8701 loci,whose mutation frequency was 44∶56.Conclusion The established method is quick and accurate for polymorphism typing of mtDNA nt8584 and nt8701 loci,and can be used for casework. |
| |
| Keywords: | mtDNA single nucleotide polymorphism fluorescent quantitative PCR |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
