STR Profiles from DNA Samples with "Undetected" or Low Quantifiler™ Results |
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| Authors: | Catherine M. Cupples M.S., Jarrod R. Champagne M.S., Kristen E. Lewis M.S., Tracey Dawson Cruz Ph.D. |
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| Affiliation: | Laboratory Corporation of America, Forensic Identity Department, 1912 Alexander Drive, Research Triangle Park, NC 27709.;Fairfax Identity Laboratories, 600 Biotech Drive, Richmond, VA 23235.;Department of Genome Science, University of Washington, Seattle, WA 98195-5065.;Departments of Forensic Science and Biology, Virginia Commonwealth University, 1000 W. Cary St., P.O. Box 842012, Richmond, VA 23284. |
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| Abstract: | Abstract: Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler™ has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/μL. Samples were analyzed once with Quantifiler™, followed by Profiler Plus™ amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler™. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler™ data may be useful for predicting STR typing success. |
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| Keywords: | forensic science DNA typing quantitative polymerase chain reaction Quantifiler™ DNA quantitation low level DNA |
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