| 应用DNA3’末端转移推测死亡时间 |
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| 作者姓名： | Zhan L Zhao ZQ Shan YW |
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| 作者单位： | 复旦大学上海医学院法医学系,上海,200032 |
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| 摘 要： | 目的根据机体死后组织细胞DNA降解规律,研究其与死亡时间之间的关系.方法SD大鼠处死后,在不同死亡时间段取肝、肾、脾组织提取DNA.根据DNA3′末端转移的方法(TdT法)将dUTP结合到DNA的3′末端,检测反应剩余的dUTP量,从而推测机体死后不同时间段DNA的降解情况.结果经对剩余dUTP检测发现,dUTP的剩余量随死亡时间延长而减少,说明DNA降解与死亡时间存在一定的关系.结论根据机体死后DNA降解规律可用于早期死亡时间的推测.
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| 关 键 词： | 脱氧核苷酸末端转移酶 死亡时间 DNA降解 |
| 文章编号： | 1004-5619(2005)02-0113-02 |
| 修稿时间： | 2005年2月1日 |
Estimation of postmortem intervals by using terminal deoxynucleotidyl transferase |
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| Zhan L,Zhao ZQ,Shan YW.Estimation of postmortem intervals by using terminal deoxynucleotidyl transferase[J].Journal of Forensic Medicine,2005,21(2):113-114. |
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| Authors: | Zhan Lan Zhao Zi-qin Shan Yi-wen |
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| Institution: | Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China. |
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| Abstract: | OBJECTIVE: To investigate the relationship between DNA degradeation and postmortem interval. METHODS: dUTP was transferred to 3'terminal of DNA by using terminal deoxynucleotidyl transferase (TDT), then the reminders of dUTP after experimental reaction, as indicator of quantity of DNA degradation, were detected. RESULTS: The reminders of dUTP were decreasing along with the postmortem interval. CONCLUSION: Postmortem DNA degradation may be used in postmortem interval judgment. |
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| Keywords: | terminal deoxynucleotidyl transferase (TdT) postmortem interval DNA degradation |
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