| 西安汉人D1S80位点基因频率调查 |
| |
| 引用本文： | 张改英,刘明俊,李生斌. 西安汉人D1S80位点基因频率调查[J]. 中国法医学杂志, 1995, 0(3) |
| |
| 作者姓名： | 张改英 刘明俊 李生斌 |
| |
| 作者单位： | 西安医科大学法医学系!西安，710061，西安医科大学法医学系!西安，710061，西安医科大学法医学系!西安，710061 |
| |
| 摘 要： | 利用PCR技术、小型聚丙烯酰胶凝胶电泳及银染法，检测D1S80位点的VNTR扩增片段长度多态性（Amp－FLP）。在175名无关的西安地区汉族人群中发现了22个等位基因，片段大小分布于320～750bp之间，频率分布为0．0057～03314，杂合度为82．3％，个人识别率（DP）为0.9588，非父排除率（EPP）为0．6704。对7个家系23名相关个体分析，证实DIS80位点的遗传符合孟德尔方式。已发现的64种基因型分布符合Hardg－Weinberg定律。
|
| 关 键 词： | D1S80 扩增片段长度多态性（Amp-FLP） 多聚酶链反应（PCR） 基因频率 |
ALLELIC FREQUENCIES OF THE D1S80 LOCUS OF THE HAN NATIONALITY IN XI''''AN |
| |
| Zhang Gaiying,et al.. ALLELIC FREQUENCIES OF THE D1S80 LOCUS OF THE HAN NATIONALITY IN XI''''AN[J]. Chinese Journal of Forensic Medicine, 1995, 0(3) |
| |
| Authors: | Zhang Gaiying et al. |
| |
| Affiliation: | Zhang Gaiying, et al. |
| |
| Abstract: | Allelic frequencies of the D1S80 locus were determined by using the PCR followed by a high-resolusion PAGE technique and silver staining. In a sample of 175 unrelated individuals of Han nationality in Xi' an, 22 alleles and 64 Renotypes were found. The distribution of genotypes was in agreement with expected values according to the Hardy-weinberg equilibrium. The helerozygosity was 82. 3% and the probability of diserimination (DP) was 0. 9588. Pedigree analysis confirmed Mendelian inheritance of the Amp-FLP alleles, and the exeluding probability of paternity (EPP) was 0. 6704. |
| |
| Keywords: | D1S80 Amp-FLP Polymerase chain reaction (PCR) Gene frequency |
| 本文献已被 CNKI 等数据库收录！ |
