多系统联合分析应用于复杂亲缘关系鉴定

目的探讨多种检测系统用于祖孙、同胞等复杂亲缘关系鉴定的效能。方法采用常染色体、性染色体STR检测系统以及常染色体STR基因座累计状态一致性评分方法筛选符合要求的参照样本,分析复杂亲缘关系鉴定中缺失被鉴定人的等位基因,对无法重建的＂空白＂等位基因,以最不利于＂认定亲缘关系＂的假设取概率值计算似然率。结果在一复杂亲缘关系鉴定案例中,从8个声称的全同胞中筛选出5个符合同父同母遗传条件的全同胞作为参照样本用于重建缺失被鉴定人等位基因,重建的父母相关基因型符合作为被检女孩亲生祖父母的遗传基因条件,祖孙亲缘关系似然率为1483659.96。结论在复杂亲缘关系鉴定中联合协同应用常染色体STR基因分型、性染色体STR基因分型、生物学全同胞关系累计状态一致性评分标准等多种分析系统有利于获得更接近真实的案件结论。

Complex Kinship Analysis by Multi-genotyping System and IBS Score

Objective To assess the efficiency of muhi-genotyping system and IBS （identity by state）score for complex kinship test. Methods The eligible reference samples were screened by multi-genotyping system and IBS Score and the data was used to reconstruct a genotype of persons who were absent in the test for some kind of reasons. The probability of those alleles that could not be reconstructed for calculating likelihood ratio （LR） of kinship was assigned on principle against the hypothesis of ＂positive＂. Results In a complex kinship test, 5 full sibs were screened out from 8 alleged full sibs as eligible reference samples to reconstruct the absent parents＇ genotype which was matched with the grandparents＇ of a alleged granddaughter in the test. The combined LR of grandparents and grandchild was 1483659.96. Conclusion Combined analysis of multi-genotyping system and IBS score could help to address more complex kinship tests.