| 荧光原位杂交技术在法庭科学DNA检验中的应用 |
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| 引用本文： | 董研,吴丹,徐庆文,张晨,平原,周怀谷. 荧光原位杂交技术在法庭科学DNA检验中的应用[J]. 中国司法鉴定, 2009, 0(4): 49-51 |
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| 作者姓名： | 董研 吴丹 徐庆文 张晨 平原 周怀谷 |
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| 作者单位： | 上海市公安局法医物证学现场应用技术,公安部重点实验室,上海,200083 |
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| 基金项目： | 上海市科学技术委员会科研计划项目 |
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| 摘 要： | 目的运用荧光原位杂交技术结合激光显微切割技术,分离法医物证男女混合样本中的男性细胞和女性细胞,并进行DNA分型。方法通过双色荧光原位杂交,Y染色体标记上绿色信号,X染色体标记上红色信号,在荧光显微镜下识别男性细胞和女性细胞,并通过激光显微切割技术分别获得男性细胞和女性细胞进行DNA分型。结果运用荧光原位杂交技术,能够分别标记法医物证男女混合样本中的男性细胞和女性细胞,并通过激光显微切割技术获得各自的DNA分型。结论荧光原位杂交技术结合激光显微切割技术,可应用于法医物证男女混合样本的检验,提高个体识别能力。
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| 关 键 词： | 荧光原位杂交 激光显微切割 法庭科学DNA检验 |
Applications of Fluorescence in Situ Hybridization(FISH) in Forensic DNA Analysis |
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| DONG Yon,WU Dan,XU Oing-wen,ZHANG Chen,PING Yuan,ZHOU Huai-gu. Applications of Fluorescence in Situ Hybridization(FISH) in Forensic DNA Analysis[J]. Chinese Journal of Forensic Sciences, 2009, 0(4): 49-51 |
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| Authors: | DONG Yon WU Dan XU Oing-wen ZHANG Chen PING Yuan ZHOU Huai-gu |
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| Affiliation: | Key Laboratory of Forensic Evidence and Scene Technology;Ministry of Public Security;Shanghai 200083;China |
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| Abstract: | Objective To prove the reliability for sex identification by detection of chromosome X and Y using fluorescence in situ hybridization and to obtain individuate DNA profiles with laser capture microdissection(LCM) from male/female mixture.Methods After fluorescence in situ hybridization, both fluorescence red signal X and green signal Y was found under a fluorescence microscope from male cells and the successful red signal of two X was observed from female cells, Combined with laser capture microdissection, ... |
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| Keywords: | FISH LCM forensic DNA analysis |
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