家兔低压电击伤后血清LDH和HBDH活性变化

目的研究家兔非热低压电击伤后不同时间乳酸脱氢酶（lactate dehydrogenase,LDH）和羟丁酸脱氢酶（hydroxybutyrate dehydrogenase,HBDH）活性的变化．为I临床和法医学非热电击伤提供诊断依据。方法健康新西兰大白兔40只,随机分为对照组和电击伤后7个时间点的实验组,每组5只。实验组使用本室已建立的非热低压电击伤方法处理,电击后各实验组动物按照预定时间点麻醉后抽取5mL心室血．测定血清LDH,HBDH酶活性。结果在非热低压电击伤后家兔目标血清酶活性发生了动态变化,有一定规律性,其中LDH活性在电击伤后4、12h和1、2、3d明显升高（4、12h和1d P〈0．01;2和3d P〈0．05）;HBDH活性在电击伤后2和3d明显升高（P〈0．05）;电击组HBD肌DH比值在2、4和12h与对照组比较显著降低（P〈0．01）。结论动态监测LDH、HBDH活性,可为非热电击损伤的诊断,损伤时间推断提供理论依据。

Dynamic changes of LDH and HBDH activity in rabbit serum after low voltage electrical injury

Objective To investigate changes of LDH and HBDH activity in rabbit serum after non-thermal low voltage electrical injury and to provide diagnostic criteria for non-thermal low voltage electrical injury.Methods Forty New Zealand rabbits were randomly distributed into control group and electrical injury group(EI-groups;designated 7 time points:0 h,2 h,4 h,12 h,1 d,2 d,3 d),5 rabbits per each group.EI-groups were treated with the method of non-thermal low voltage electrical injury established in our laboratory.Ventricular blood(5 mL) was obtained under anesthesia at designated time points after electrical injury.The activities of LDH and HBDH were measured.Results Dynamic changes were observed with certain patterns from target serum enzyme activities after electrical injury.Compared with control group,the activities of LDH increased markedly at 4 h,12 h,and on days 1,2,and 3 after injury(4 h,12 h,and day 1 P<0.01;day 2 and day 3 P<0.05).Activities of HBDH increased markedly at day 2 and day 3 after injury(P<0.05).The ratio of HBDH/LDH decreased markedly at 2 h,4 h,and 12 h after injury(P<0.01).Conclusion Dynamic changes of LDH and HBDH activities may be useful in diagnosis of non-thermal low voltage electrical injury and in estimation of post injury intervals.