| 食品中沙门氏菌DNA环介导等温扩增快速检测方法的建立 |
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| 引用本文： | 徐义刚,崔丽春,杨君宏,李苏龙,李丹丹,姜艳春,张子群. 食品中沙门氏菌DNA环介导等温扩增快速检测方法的建立[J]. 中国兽医科学, 2010, 40(5) |
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| 作者姓名： | 徐义刚 崔丽春 杨君宏 李苏龙 李丹丹 姜艳春 张子群 |
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| 作者单位： | 徐义刚,李苏龙,姜艳春,张子群,XU Yi-gang,LI Su-long,JIANG Yan-chun,ZHANG Zi-qun(黑龙江出入境检验检疫局,检验检疫技术中心,黑龙江,哈尔滨,150001);崔丽春,CUI Li-chun(东北林业大学,黑龙江,哈尔滨,150040);杨君宏,YANG Jun-hong(哈尔滨市粮食研究所,黑龙江,哈尔滨,150076);李丹丹,LI Dan-dan(海南出入境检验检疫局,检验检疫技术中心,海南,海口,570125) |
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| 基金项目： | 国家质检总局科技计划项目，黑龙江省博士后基金 |
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| 摘 要： | 根据沙门氏菌属高度保守的fimY基因序列设计了2对特异性引物,采用环介导等温扩增方法(LAMP)建立了食品中沙门氏菌的LAMP快速检测方法。结果表明,建立的LAMP方法具有高度特异性,经对40种共68株细菌进行扩增,所试32株沙门氏菌均为LAMP阳性,其他菌株为LAMP阴性。建立的LAMP方法对培养的沙门氏菌的检测灵敏度为5CFU/管,对污染食品中沙门氏菌的检测灵敏度为9CFU/管,60min内即可完成检测。用建立的LAMP方法对802份肉类、蛋、奶及其制品和动物腹泻物、人工污染样品等进行检测,共检出165份LAMP阳性样本,与国标(GB/T4789.4-2008)方法的检测结果一致。建立的LAMP方法操作简便、特异性强、灵敏度高,具有良好的实用性。
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| 关 键 词： | 沙门氏菌 fimY基因 环介导等温扩增 快速检测 |
Development of a loop-mediated isothermal amplification method with fimY gene for rapid detection of Salmonella in food |
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| XU Yi-gang,CUI Li-chun,YANG Jun-hong,LI Su-long,LI Dan-dan,JIANG Yan-chun,ZHANG Zi-qun. Development of a loop-mediated isothermal amplification method with fimY gene for rapid detection of Salmonella in food[J]. Chinese Veterinary Science, 2010, 40(5) |
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| Authors: | XU Yi-gang CUI Li-chun YANG Jun-hong LI Su-long LI Dan-dan JIANG Yan-chun ZHANG Zi-qun |
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| Affiliation: | XU Yi-gang1,CUI Li-chun2,YANG Jun-hong3,LI Su-long1,LI Dan-dan4,JIANG Yan-chun1,ZHANG Zi-qun1 (1.Technical Centre of Heilongjiang Entry-Exit Inspection , Quarantine Bureau,Harbin 150001,China,2.Northeast Forestry University,Harbin 150040,3.Harbin Grain Research Institute,Harbin 150076,4.Technical Centre of Hainan Entry-Exit Inspection , Quarantine Bureau,Haikou 570125,China) |
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| Abstract: | According to the highly conserved fimY gene of Salmonella,two pairs of primers were designed and the method for detecting Salmonella in food was developed with loop-mediated isothermal amplification(LAMP).In results,sixty-eight strains of forty bacteria species were used for specificity test and all the tested Salmonella strains were positive and the other strains were negative in LAMP,indicating that the LAMP method was highly specific for target bacteria with the sensitivity of 5 CFU per test tube for cul... |
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| Keywords: | Salmonella fimY gene loop-mediated isothermal amplification rapid detection |
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