| 甲醛固定及石蜡包埋组织的DNA分型及其应用 |
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| 作者姓名： | Lin Y Zheng SG Lu XY Que TZ Luo JM Xiao L |
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| 作者单位： | 司法部司法鉴定科学技术研究所,上海医科大学附属肿瘤医院,上海华东医院 |
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| 摘 要： | 目的：建立甲醛固定及石蜡包埋组织ＤＮＡ的基因分型方法并使之应用于实际检案。方法：用Ｃｈｅｌｅｘ和有机溶剂提取法提取ＤＮＡ,以ＰＣＲ与反向探针杂交技术作ＰＭ和ＨＬＡ－ＤＱＡ１位点分型。结果：２６份包埋组织块有２４份能得到理想的分型结果。结论：本法可用于法医学个人识别和亲子鉴定
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| 关 键 词： | PCR复合扩增 反向杂交技术 甲醛固定 石蜡包埋组织 |
Research on DNA typing in the forensic identification of formalin-fixed paraffin embedded tissues |
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| Lin Y,Zheng SG,Lu XY,Que TZ,Luo JM,Xiao L.Research on DNA typing in the forensic identification of formalin-fixed paraffin embedded tissues[J].Journal of Forensic Medicine,1999,15(2):82-83. |
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| Authors: | Lin Y Zheng S G Lu X Y Que T Z Luo J M Xiao L |
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| Institution: | Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, P. R. China. |
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| Abstract: | Successful extraction of DNA from
formalin-fixed Paraffin embedded tissues is important to DNA typing for forensic purpose.In the
research,26 samples were analyzed by method of PM and HLA-DQA1 loci typing.24 of these
samples were successful.It showed that this technique and method could be applied to individual
identification and paternity test by use of these sorts of samples. |
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| Keywords: | PCR multiplex amplification reverse dot blot formalin-embedded tissue |
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