迟发性外伤性脑内血肿脑组织GFAP、S-100和NSE的免疫组织化学研究

应用免疫组织化学ABC法和S－P法，对6例伤后1．5天至2年发生的迟发性外伤性脑内血肿（DTICH）脑组织标本进行神经胶质细胞内胶质纤维酸性蛋白（GFAP）、S－100蛋白（S－100）和神经元细胞内特异性烯醇化酶（NSE）变化和应用价值的研究。尸检和组织学检查发现有脑挫伤等病变。6例DTICH脑组织挫伤及周围区均有明显的GFAP、S－100阳性的细胞变化，细胞数目增多，胞体肥大、增生，免疫组化反应产物增多、深染；神经元细胞NSE免疫组化染色均呈阴性改变。结果表明：6例DTICH脑组织中GFAP、S－100的NSE的免疫组织化学变化是有一定时间间隔的外伤性陈旧性改变，在DTICH的病理诊断上具有重要价值，可作为DTICH法医学鉴定的一个辅助手段。

Immunohistochemical study of GFAP,S-100 and NSE in brain tissues of delayed traumatic intracerebral hematoma

Using immunohistochemical technique (ABC and S-P method), we studied the changes of glial fibrillary acid protein (GFAP), S-100 protein (S-100) and neuron-specif ic enolase (NSE) of brain tissues of 6 cases died of delayed traumatic intracerebral hematoma (DTICH) which occured from 1. 5 days to 2 years after trauma. The results showed that there were brain contussions etc. in 6 cases by autopsy and histological examination. There were remarkably immunohistochemical changes , that is, increased number,hypertrophy and proliferation of glia, increase and deep staining of immunohistochemical products of GFAP-positive astrocytes and S-100-positive glia in brain contussions and their peripheral areas, and no NSE-positive neurons were stained in contussion areas. These findings indicated that immunohistochemical changes of GFAP , S- 100 and NSE in 6 cases were the characteristic changes of the old brain trauma, which has important value in diagnosis of DTICH, and could be served as subsidiary diagnostic method of forensic expertise of DTICH.