| 利用压力循环仪提取骨骼DNA |
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| 引用本文： | 苑美青,徐政,李彩霞,胡兰,李万水.利用压力循环仪提取骨骼DNA[J].刑事技术,2011(4):29-32. |
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| 作者姓名： | 苑美青 徐政 李彩霞 胡兰 李万水 |
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| 作者单位： | 公安部物证鉴定中心法医遗传学公安部重点实验室,北京,100038 |
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| 基金项目： | 公安部应用创新计划资助项目(2009YYCXGAES128) |
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| 摘 要： | 目的探讨建立利用压力循环技术提取骨骼DNA的新方法。方法将11个不同部位骨骼样品分成3组,一组采用本实验室常规方法处理骨骼,一组采用压力循环仪处理骨骼,另外一组作为阴性对照,然后统一进行DNA的提取及定量。结果采用压力循环技术提取骨骼DNA时间比实验室常规方法缩短24小时以上,提取效率提高6%～49%。结论压力循环技术可以作为一种提取骨骼DNA提取的新方法之一。
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| 关 键 词： | 骨骼 压力循环技术 DNA提取 |
A rapid and effective bone DNA extraction method using pressure cycling technology |
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| YUAN Mei-qing,TU Zheng,LI Cai-xia,et al.A rapid and effective bone DNA extraction method using pressure cycling technology[J].Forensic Science and Technology,2011(4):29-32. |
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| Authors: | YUAN Mei-qing TU Zheng LI Cai-xia |
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| Institution: | YUAN Mei-qing,TU Zheng,LI Cai-xia,et al(Institute of Forensic Sciences,Ministry of Public Security,Beijing 100038,China) |
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| Abstract: | Objective To establish a new method for DNA extraction from bone samples.Methods DNA of 11 bone samples was extracted and quantificated by using pressure cycling technology.Results Compared to traditional protocol,the pressure cycling technology greatly streamlined bone DNA extraction process,and DNA recovery rate increased from 6% to 49%.Conclusion Pressure cycling technology was proved as one of effective bone DNA extraction method in forensic application. |
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| Keywords: | bones pressure cycling technology DNA extraction |
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