针刺上巨虚穴对慢传输型便秘小鼠结肠组织Cajal间质细胞数的影响

目的 探讨针刺上巨虚穴治疗慢传输型便秘（slow transit constipation，STC）的机制。方法 将40只小鼠分为正常组、模型组、上巨虚组（针刺双侧上巨虚）、非经非穴组（针刺小鼠臀部两处非经非穴点），每组各10只。采用复方地芬诺酯灌胃法复制小鼠STC模型。观察各组首次排便时间、12 h内排便粒数，采用免疫组织化学方法观察各组小鼠结肠Cajal间质细胞(interstitial cells of Cajal，ICC)数量。结果 与正常组比较，模型组小鼠的首次排便时间明显延长 （P<0.01），12 h内排便粒数和结肠ICC数量明显减少（P<0.01）；与模型组比较，上巨虚组小鼠的首次排便时间明显缩短（P<0.01），12 h内排便粒数和结肠ICC数量明显增多（P<0.01）。结论 针刺上巨虚穴对STC具有明显的改善作用，其作用机制可能与升高结肠组织ICC数量有关。

Effect of Acupuncture at Shangjuxu (ST 37) on Number of Colonic Interstitial Cells of Cajal in Mice with Slow Transit Constipation

Objective To investigate the mechanism of acupuncture at Shangjuxu (ST 37) in the treatment of slow transit constipation (STC). Methods Forty mice were divided into normal group (n=10), model group (n=10), ST 37 group (n=10), and non-acupuncture point group ( n =10). All rats except those in normal group received compound diphenoxylate by gavage to induce a mouse model of STC. The ST 37 group received acupuncture at bilateral ST 37, while the non-acupuncture point group received acupuncture at two non-acupuncture points on the hip. The time to first defecation and number of feces granules within 12 h were determined. The number of colonic interstitial cells of Cajal (ICC) was determined by immunohistochemistry. Results Compared with the normal group, the model group had significantly prolonged time to first defecation (P<0.01) and significantly reduced number of feces granules within 12 h and number of colonic ICC ( P<0.01). Compared with the model group, the ST 37 group had significantly shortened time to first defecation (P<0.01) and significantly increased number of feces granules within 12 h and number of colonic ICC ( P<0.01). Conclusion Acupuncture at ST 37 has significant efficacy in the treatment of STC, possibly by increasing the number of colonic ICC.