温针灸、谢氏点穴及其联合治疗对高脂饮食诱导的膝骨关节炎大鼠的作用机制研究

目的 探讨温针灸、谢氏点穴及其联合治疗对肥胖大鼠膝骨关节炎的治疗效果与作用机制.方法 采用高脂饮食诱导肥胖大鼠膝骨关节炎,检测温针灸、谢氏点穴以及温针灸+谢氏点穴治疗后肥胖大鼠体质量,血清总胆固醇(total cholesterol,TC)、三酰甘油(total triglyceride,TG)、高密度脂蛋白(high...

Mechanism of Action of Warm Acupuncture and Xie s Acupoint Therapy Used Alone or in Combination in Treatment of Rats with Knee Osteoarthritis Induced by High-fat Diet

Objective To investigate the therapeutic effect and mechanism of action of warm acupuncture and Xie s acupoint therapy used alone or in combination in the treatment of obese rats with knee osteoarthritis.Methods High-fat diet was used to induce knee osteoarthritis in obese rats,and related indices were measured after warm acupuncture alone,Xie s acupoint therapy alone,and warm acupuncture+Xie s acupoint therapy,including body weight,serum levels of blood lipids[total cholesterol(TC),total triglyceride(TG),high-density lipoprotein(HDL),and low-density lipoprotein(LDL)],knee joint lesions,the content of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein-1(MCP-1),and vascular endothelial growth factor(VEGF)in synovial fluid,and the levels of matrix metalloproteinase-1(MMP-1)and matrix metalloproteinase-13(MMP-13)in the cartilage layer of the knee joint.Results Compared with the control group,the model group had significant increases in body weight,the serum levels of TC,TG,and LDL,Mankin score of the knee joint,the levels of IL-1β,TNF-α,MCP-1,and VEGF in synovial fluid,and the expression levels of MMP-1 and MMP-13 in the cartilage layer of the knee joint(P<0.05),and a significant decrease in serum level of HDL(P<0.05).Compared with the model group,the treatment groups had significant reductions in the serum levels of TC and TG,Mankin score of the knee joint,the levels of MCP-1 and VEGF in synovial fluid,and the expression level of MMP-13 in the cartilage layer of the knee joint(P<0.05).Xie s acupoint therapy and warm acupuncture+Xie s acupoint therapy significantly increased the serum level of HDL,reduced the level of TNF-αin synovial fluid,and the expression of MMP-1 in the cartilage layer of the knee joint;warm acupuncture+Xie s acupoint therapy significantly reduced the serum level of LDL and the level of IL-1βin synovial fluid(P<0.05);compared with the warm acupuncture group and the Xie s acupoint therapy group,the warm acupuncture+Xie s acupoint therapy group had significantly better reductions in the serum levels of TC,TG,and LDL,Mankin score of the knee joint,and the expression levels of MMP-1 and MMP-13 in the cartilage layer of the knee joint(P<0.05).Conclusion Warm acupuncture combined with Xie s acupoint therapy can alleviate obesity-induced knee osteoarthritis by regulating lipid metabolism and reducing the secretion of VEGF and MCP-1 in synovial fluid.