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正交设计优选百蕊草的提取工艺
引用本文:储晓琴,岳 磊,胡叶青,谢法平. 正交设计优选百蕊草的提取工艺[J]. 安徽中医药大学学报, 2013, 32(2): 80-82
作者姓名:储晓琴  岳 磊  胡叶青  谢法平
作者单位:安徽中医学院药学院,安徽,合肥,230031;现代中药安徽省重点实验室,安徽,合肥,230031;安徽中医学院药学院,安徽,合肥,230031
摘    要:目的 优选百蕊草的最佳提取工艺。方法 采用正交试验法,以干膏得率及山奈酚含量为指标,考察溶剂浓度、溶剂用量、提取时间以及提取次数的影响,确定最佳提取工艺。结果 最佳提取工艺参数为:加8倍量80%乙醇,提取3次,每次1 h。结论 优选的提取工艺切实可行,可以作为百蕊草的提取工艺。

关 键 词:百蕊草  山奈酚  正交设计

Optimization of Extraction Process of Thesium chinense Turcz. by Orthogonal Design
Affiliation:1.School of Pharmacy,Anhui University of Traditional Chinese Medicine,Anhui Hefei 230031, China;2.Anhui Key Laboratory of Modern Chinese Medicine,Anhui Hefei 230031,China
Abstract:Objective To optimize the extraction process of Thesium chinense Turcz. Methods The orthogonal design was used to investigate the effects of solvent concentration, solvent volume, extraction time, and extraction frequency on yield of dry extract and content of kaempferol (as assessment indices) and to determine the optimal extraction process. Results In the optimal process, extraction was performed 3 times (for 1 h each time) using 80% ethanol (8 times). Conclusion The optimized extraction process is feasible and reliable, and it can be used for extracting Thesium chinense Turcz.
Keywords:Thesium chinense Turcz.   kaempferol   orthogonal design
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