瓜蒌薤白汤对博来霉素致大鼠肺纤维化内质网应激反应的影响

Effect of Gualou Xiebai Decoction on Endoplasmic Reticulum Stress in Rats with Bleomycin-induced Pulmonary Fibrosis

Objective To investigate the effect of Gualou Xiebai Decoction on endoplasmic reticulum stress in rats with bleomycin-induced pulmonary fibrosis,and to clarify the mechanism of action of Gualou Xiebai Decoction on bleomycin-induced pulmonary fibrosis.Methods A total of 40 rats were randomly divided into normal control group,model group,positive control group,and Gualou Xiebai Decoction group,with 10 rats in each group.A rat model of pulmonary fibrosis was established by bleomycin injection.Since the next day,the rats in the positive control group and Gualou Xiebai Decoction group were given the corresponding drugs by gavage,once a day for 28 consecutive days.On day 28 after the last administration,the rats were sacrificed by exsanguination from the femoral artery after anesthesia.Lung tissue was harvested to measure the weight and perform gross observation,and the lung coefficient was calculated.The lower left lung was placed in 4%paraformaldehyde for fixation,and Masson staining and HE staining were performed.Immunohistochemistry was used to measure the expression of p-PERK,p-IRE1α,GRP78,and ATF6αin lung tissue.Results The model group had a significantly higher lung coefficient than the other three groups(P<0.05),and the positive control group had a significantly higher lung coefficient than the Gualou Xiebai Decoction group(P<0.05).Compared with the model group,the positive control group and the Gualou Xiebai Decoction group had a significant reduction in the degree of inflammation(P<0.05),while there was no significant difference in the degree of inflammation between these two groups and the normal control group(P>0.05).The model group had a significantly higher degree of pulmonary fibrosis than the positive control group and the Gualou Xiebai Decoction group(P<0.05).Compared with the normal control group,the model group had significant increases in the expression of p-PERK,p-IRE1α,GRP78,and ATF6αin lung tissue(P<0.05),while there were no significant differences in their expression between the positive control group/Gualou Xiebai Decoction group and the normal control group(P>0.05).Conclusion Gualou Xiebai Decoction can significantly inhibit bleomycin-induced pulmonary fibrosis in rats,possibly by inhibiting endoplasmic reticulum stress.