利用GSS高通量测序技术分析不同生境下霍山石斛内生真菌群落的分布

目的 研究不同地域及不同生长环境下霍山石斛内生真菌群落的分布特征。方法 采用DNA分子标定技术确定不同霍山石斛样本的属种，利用GSS高通量测序技术分析霍山石斛样本内生真菌的种类。结果 供试的所有来源的霍山石斛样本均为Dendrobium huoshanense。从10个霍山石斛样本（编号LP1、LP2、LD1、LD2、LS1、LS2、HP1、HP2、JP1、JP2）中分别获得OTU数540、390、405、376、121、601、423、426、392、394个，通过分析每个OTU对应的物种分类信息，这些霍山石斛样本的内生真菌群落分布可归属至种水平，且分别包含内生真菌140、110、134、128、133、142、144、127、151、135种；样本LP1和LP2共有的OTU在物种水平上占84.22%，LD1和LD2共有的OTU在物种水平上占95.58%，LS1和LS2共有的OTU在物种水平上占94.52%，HP1和HP2共有的OTU在物种水平上占93.59%，JP1和JP2共有的OTU在物种水平上占94.05%。结论 不同地域及不同生长环境下霍山石斛（Dendrobium huoshanense）的内生真菌种类存在着差异性，相同地域及相同生长环境下霍山石斛（Dendrobium huoshanense）的内生真菌群落组成基本相同，霍山石斛（Dendrobium huoshanense）内生真菌的生长存在着地域专属性。

Distribution of Endophytic Fungi in Dendrobium huoshanense from Different Habitats by Green Shield Sequencing

Objective To investigate the distribution characteristics of endophytic fungi in Dendrobium huoshanense from different habitats. Methods The DNA molecular calibration technique was used to identify the species of different Dendrobium huoshanense samples, and Green Shield Sequencing was used to analyze the distribution of endophytic fungi in Dendrobium huoshanense. Results All test samples were identified as Dendrobium huoshanense. The operational taxonomic unit (OTU) numbers of 10 samples of Dendrobium huoshanense (LP1, LP2, LD1, LD2, LS1, LS2, HP1, HP2, JP1, and JP2) were 540, 390, 405, 376, 121, 601, 423, 426, 392, and 394, respectively. Through an analysis of the species taxonomy of each OTU, the distribution of endophytic fungi in Dendrobium huoshanense could be attributed to the species level, and the numbers of the species of endophytic fungi in these samples were 140, 110, 134, 128, 133, 142, 144, 127, 151, and 135, respectively. The OTUs shared by LP1 and LP2, LD1 and LD2, LS1 and LS2, HP1 and HP2, and JP1 and JP2 accounted for 84.22%, 95.58%, 94.52%, 93.59%, and 94.05%, respectively, on the species level. Conclusion There are differences in the species of endophytic fungi between Dendrobium huoshanense from different habitats. Dendrobium huoshanense samples from the same habitat have a similar community composition of endophytic fungi, and the growth of endophytic fungi in Dendrobium huoshanense has regional specificity.