表没食子儿茶素没食子酸酯诱导人肝癌细胞HCCLM6凋亡的研究

目的 观察表没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)对人高转移肝癌细胞HCCLM6凋亡的作用及探讨其作用机制。方法 甲基噻唑基四唑染色法检测EGCG对人肝癌细胞HCCLM6细胞增殖的抑制作用，流式细胞术检测细胞凋亡率和EGCG对HCCLM6细胞周期的影响，免疫荧光技术检测EGCG处理的HCCLM6细胞中血管内皮生长因子(vascular endothelial growth factor, VEGF)和骨桥蛋白(osteopontin, OPN)表达的变化。结果 EGCG可显著抑制肝癌细胞HCCLM6的增殖，且呈浓度依赖性；EGCG使细胞周期明显阻滞于G0/G1期；EGCG可明显导致HCCLM6细胞凋亡，使VEGF、OPN表达水平显著下降。结论 EGCG可以诱导肝癌HCCLM6细胞凋亡，其作用机制可能与阻滞细胞周期和调控VEGF、OPN表达有关。

Pro-apoptotic Effect of Epigallocatechin Gallate on Human Hepatocellular Carcinoma Cell Line HCCLM6

Objective To observe the pro-apoptotic effect of epigallocatechin gallate (EGCG) on human hepatocellular carcinoma cell line HCCLM6, which has a high metastatic potential, and to investigate its action mechanism. Methods MTT assay was used to evaluate the inhibitory effect of EGCG on the proliferation of HCCLM6 cells. Flow cytometry was used to measure the apoptosis rate of HCCLM6 cells treated with EGCG and evaluate the effect of EGCG on the cell cycle of HCCLM6 cells. The expression of vascular endothelial growth factor (VEGF) and osteopontin (OPN) in EGCG-treated HCCLM6 cells was measured by immunofluorescence technique. Results EGCG significantly inhibited the proliferation of HCCLM6 cells in a concentration-dependent manner (P<0.01). EGCG significantly increased the apoptosis rate of HCCLM6 cells (P<0.05) and significantly arrested HCCLM6 cells in G0/G1 phase (P<0.01). The expression of OPN and VEGF in EGCG-treated HCCLM6 cells was significantly reduced (P<0.01). Conclusion EGCG can induce apoptosis of HCCLM6 cells, which may be due to that EGCG arrests cell cycle progression and regulates the expression of VEGF and OPN.