艾灸干预大鼠类风湿关节炎的尿液代谢组学研究

目的 基于代谢组学技术研究类风湿关节炎（rheumatoid arthritis, RA）模型大鼠尿液中内源性代谢物的变化,初步探索艾灸干预RA的作用机制。方法 将80只大鼠随机分成正常对照组、模型对照组、雷公藤多苷片（Tripterygium wilfordii polyglycoside tablet, TPT）组和艾灸组,每组20只。采用右后足跖皮内注射弗氏完全佐剂的方法复制RA大鼠模型。干预15 d后，采用气相色谱-质谱联用仪技术检测尿液代谢组学，利用主成分分析和偏最小二乘法判别分析对数据进行统计分析,观察大鼠尿液中内源性代谢物的变化。结果 与正常对照组比较，模型对照组的变化体现于色氨酸、对羟基肉桂酸、对羟基苯丙酸、氨基丁酸、甲基胞嘧啶、喹啉酸和脱氧核糖水平降低，油酸和胆固醇水平升高；与模型对照组比较，艾灸组的变化主要体现于氨基丁酸、甲基胞嘧啶、喹啉酸、脱氧核糖水平升高；而TPT组较模型对照组的变化主要体现于胆固醇水平降低，油酸、对羟基肉桂酸和羟基苯丙酸水平升高；与艾灸组大鼠比较，TPT组的变化主要体现于胆固醇、甲基胞嘧啶、喹啉酸、氨基丁酸、脱氧核糖水平升高。结论 艾灸及TPT均可使RA模型大鼠尿液异常的氨基酸代谢、嘧啶代谢、脂质代谢等发生逆转，而艾灸治疗RA的特异性可能体现在氨基酸代谢调控方面。

Mechanism of Action of Moxibustion in Intervention of Rheumatoid Arthritis in Rats: A Study Based on Urine Metabolomics

Objective To investigate the changes in urine endogenous metabolites in rats with rheumatoid arthritis (RA) using the metabolomics technology, as well as the mechanism of action of moxibustion in the intervention of RA. Methods A total of 80 rats were randomly divided into normal control group, model control group, Tripterygium wilfordii polyglycoside tablet (TPT) group, and moxibustion group,with 20 rats in each group. A rat model of RA was established by intradermal injection of Freund''s complete adjuvant at the right rear vola pedis. After 15 days of intervention, gas chromatography-mass spectrometry was used to detect urine metabolomics, the principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used for the statistical analysis of data, and the changes in urine endogenous metabolites were observed. Results Compared with the normal control group, the model control group had reductions in tryptophan, hydroxy cinnamic acid, hydroxy benzoic acid, aminobutyric acid, methyl cytosine, quinolinic acid, and desoxyribose and increases in oleic acid and cholesterol. Compared with the model control group, the moxibustion group had increases in aminobutyric acid, methyl cytosine, quinolinic acid, and desoxyribose, and the TPT group had a reduction in cholesterol and increases in oleic acid, hydroxy cinnamic acid, and hydroxy benzoic acid. Compared with the moxibustion group, the TPT group had increases in cholesterol, methyl cytosine, quinolinic acid, amino butyric acid, and desoxyribose. Conclusion Both moxibustion and TPT can reverse the abnormal metabolism of amino acids, pyrimidine, and lipids in RA rats, and the regulation of amino acid metabolism reflects the specificity of moxibustion in the treatment of RA.