| 可见分光光度法测定覆盆子中总三萜含量 |
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| 作者姓名： | 郭宣宣 邱晓霞 张玲 曹富 岳婧怡 董志雨 |
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| 作者单位： | 安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥,230012;安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥,230012;安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥,230012;安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥,230012;安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥,230012;安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥,230012 |
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| 基金项目： | 安徽省高校自然科学研究重点项目（KJ2015A128） |
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| 摘 要： | 目的 建立覆盆子中总三萜的含量测定方法。方法 以齐墩果酸为对照品，香草醛-冰醋酸-高氯酸为显色体系，采用可见分光光度法在480 nm处测定覆盆子中总三萜的含量。〖JP〗结果 齐墩果酸在0.079 60～0.398 0 mg范围内与吸光度呈良好的线性关系，回归方程为y＝2.117x＋0.005 380（r＝0.999 7），平均加样回收率为101.92%(RSD＝1.92%，n＝6)。结论 该方法操作简便、快速、灵敏度高、重现性好、测定结果准确，可作为覆盆子中总三萜含量测定的方法。
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| 关 键 词： | 覆盆子 总三萜 齐墩果酸 可见分光光度法 |
Determination of Total Triterpenoids in Rubi Fructus by Visible Spectrophotometry |
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| Institution: | School of Pharmacy, Anhui University of Chinese Medicine & Anhui Key Laboratory of Modern Chinese Materia Medica, Anhui Hefei 230012, China |
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| Abstract: | Objective To establish a method for determining total triterpenoids in Rubi Fructus. Methods With oleanolic acid as the reference substance and vanilline-glacial acetic acid-perchloric acid as the color-developing agents, visible spectrophotometry was used to determine total triterpenoids in Rubi Fructus at a wavelength of 480 nm. Results Oleanolic acid showed a good linear relationship with absorbance within the range of 0.079 60-0.398 0 mg, with a regression equation of y = 2.117x+0.0053 80 (r=0.999 7) and an average recovery rate of 101.92% (relative standard deviation=1.92%, n=6). Conclusion This method is simple, rapid, highly sensitive, reproducible, and accurate and can be used for the determination of total triterpenoids in Rubi Fructus. |
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| Keywords: | Rubi Fructus Total triterpenes Oleanolic acid Visible spectrophotometry |
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