电针胃俞募配穴对胃扩张模型大鼠海马c-fos表达水平及神经细胞活动的影响

目的 探讨海马参与电针胃俞募配穴调节胃扩张模型大鼠胃运动的中枢机制.方法 将40只7周龄SD大鼠随机分为模型组、胃俞组、中脘组、中脘+胃俞组、非经非穴组,每组8只.采用胃内球囊扩张法复制胃扩张模型.模型组不予针刺,其余各组进行电针干预,每日1次,每次20 min,连续干预7 d.采用压力换能器检测大鼠胃内压,用双导智能胃肠电图仪测定大鼠体表胃电.采用免疫荧光法检测大鼠海马c-fos的表达水平,采用微阵列电极技术记录大鼠海马神经细胞放电变化.结果 与模型组比较,中脘组、胃俞组及中脘+胃俞组大鼠胃内压、胃电振幅均显著升高(P<0.05),海马CA1区c-fos表达水平及海马CA1区神经细胞放电频率均显著增加(P<0.05),但非经非穴组上述指标均无明显变化(P>0.05).与中脘组、胃俞组比较,中脘+胃俞组大鼠胃内压、胃电振值均显著上升(P<0.05),海马CA1区c-fos表达水平显著增加(P<0.05),海马CA1区神经细胞放电频率显著增加(P<0.05).结论 海马CA1区神经细胞参与电针胃俞募配穴对胃运动的调节机制.

Effect of Electroacupuncture at Gastric Shu-Mu Points on c-fos Expression and Neural Cell Activity in the Hippocampus of Rats with Gastric Distension

Objective To investigate the central mechanism of the hippocampus in regulating gastric motility in a rat model of gastric distension by electroacupuncture at gastric Shu-Mu points. Methods A total of 40 Sprague-Dawley rats, aged 7 weeks, were randomly divided into model group, Weishu group, Zhongwan group, Zhongwan+Weishu group, and non-meridian/acupoint group, with 8 rats in each group. Gastric balloon dilatation was performed to establish a rat model of gastric distension. The rats in the model group were not given acupuncture, and those in the other groups were given electroacupuncture once a day, 20 minutes each time, for 7 consecutive days. A pressure transducer was used to measure intragastric pressure, and a double-channel intelligent gastrointestinal electrograph was used to obtain surface electrogastrogram. Immunofluorescence assay was used to measure the expression level of c-fos in the hippocampus, and the microarray electrode technique was used to record the change in the discharge of hippocampal neural cells. Results Compared with the model group, the Zhongwan group, the Weishu group, and the Zhongwan+Weishu group had significant increases in the amplitudes of intragastric pressure and gastric electrical activity (P<0.05), the expression of c-fos in the hippocampal CA1 region, and neuronal discharge frequency in the hippocampal CA1 region (P<0.05), while the non-meridian/acupoint group had no significant changes in the above indicators (P>0.05). Compared with the Zhongwan group and the Weishu group, the Zhongwan+Weishu group had significant increases in the amplitudes of intragastric pressure and gastric electrical activity (P<0.05), the expression of c-fos in the hippocampal CA1 region (P<0.05), and neuronal discharge frequency in the hippocampal CA1 region (P<0.05). Conclusion The hippocampal CA1 neurons are involved in the mechanism of electroacupuncture at gastric Shu-Mu points in regulating gastric motility.