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Typing study of human semen DIA3 by isoelectric focusing--distribution in the Wuhan population, China
Authors:Q G Yange  Q J Huang  R Z Yang  K Mei
Affiliation:Department of Forensic Serology, Faculty of Forensic Medicine, Tong Ji Medical University, Wuhan, People's Republic of China.
Abstract:Human semen DIA3 typing was studied by isoelectric focusing on ultra-thin-layer polyacrylamide gel which resulted in a simpler and more definite separation of the products of DIA3 alleles than hitherto. In 198 semen samples collected from unrelated Chinese males four different phenotypes were observed. The DIA3 allele frequencies were calculated: DIA 3(1) = 0.7727, DIA 3(2) = 0.2172, DIA 3(3) = 0.0101. The results of the stability study of 12 laboratory-prepared semen stains stored at room temperature suggested that DIA3 in seminal strains is a relatively stable genetic marker. Our gene frequencies have been compared to those reported in other populations.
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