| 串联亲和纯化中应用不同标签组合的研究进展 |
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| 引用本文： | 魏衍全,孙世琪,孙德惠,郭慧琛. 串联亲和纯化中应用不同标签组合的研究进展[J]. 中国兽医科学, 2012, 0(9): 987-990 |
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| 作者姓名： | 魏衍全 孙世琪 孙德惠 郭慧琛 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室 |
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| 基金项目： | 甘肃省科技重大专项(1102NKDA033,1102NKDA034);国家自然科学基金资助项目(31100688,31101838);甘肃省国际科技合作计划项目(1104WCGA185) |
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| 摘 要： | 综述了原始串联亲和纯化方法的原理及其存在的缺陷和各种最新构建的串联亲和纯化标签组合及其从哺乳动物细胞中纯化蛋白复合物时的蛋白得率。与单步纯化方法相比,串联亲和纯化方法显著地减少了非特异性背景,可分离到高纯度的蛋白复合物。这一优势极大地简化了蛋白质鉴定及其相互作用关系的验证工作。
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| 关 键 词： | 串联亲和纯化 亲和标签 蛋白复合体 高蛋白得率 |
Progress on different combinations of affinity tags in tandem affinity purification |
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| WEI Yan-quan,SUN Shi-qi,SUN De-hui,GUO Hui-chen. Progress on different combinations of affinity tags in tandem affinity purification[J]. Chinese Veterinary Science, 2012, 0(9): 987-990 |
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| Authors: | WEI Yan-quan SUN Shi-qi SUN De-hui GUO Hui-chen |
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| Affiliation: | (State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Animal Virology of the Ministry of Agriculture/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | The paper reviews the principle and limitations in the original tandem affinity purification(TAP) method,the newly developed TAP tags and their high recovery of protein complexes from mammalian cells.Compared with single-step purification,TAP method greatly reduces non-specific background and isolates protein complexes with higher purity.This dominant feature greatly simplifies identification and validation of the isolated proteins as true interacting partners. |
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| Keywords: | tandem affinity purification affinity tag protein complex high recovery |
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