GnRH受体在山羊颈中神经节的分布特点

为了证实GnRH受体在山羊颈中神经节是否存在,取雄性和雌性成年山羊的颈中神经节各5对,经免疫组织化学SP法染色后,观察GnRH受体在颈中神经节的分布特点。结果表明,山羊颈中神经节的神经元、血管内皮细胞、神经纤维及卫星细胞均有不同程度的阳性染色。神经元中GnRH受体强阳性产物分布于胞膜上,中等阳性产物分布于整个胞质中;血管内皮细胞有中等阳性产物;神经纤维、卫星细胞和施万细胞有弱阳性产物分布。证实,GnRH受体在山羊颈中神经节中分布广泛,提示颈中神经节可能受GnRH调节,这为研究GnRH非生殖调节功能及颈中神经节的神经内分泌调节机制提供了形态学依据。

Distribution characteristics of GnRH receptor in the middle cervical ganglion of goat

This experiment was conducted to detect the existence of GnRH receptor(GnRHR) in the middle cervical ganglion(MCG) of goat.Five pairs of MCG were taken from mature female and male goats,respectively.The distribution characteristics of GnRHR were observed after immunohistochemical SP staining.The results showed that:for neurons,strong GnRHR immunoreactivity(GnRHR-ir) and weak GnRHR-ir products distributed on the membrane and the whole cytoplasm,respectively;the vascular endothelial cells were moderate positive;while the nerve fibers,the satellite cells and the Schwan cells were weak positive.The wide distribution of GnRHR in MCG implied that GnRH may regulate MCG,which provided morphologic basis for studies on non-reproductive regulating function of GnRH and neuroendocrine regulation of MCG.