脑干损伤后神经轴突和髓鞘变化的免疫组织化学研究

探讨脑干损伤后神经轴突和髓鞘的病理学改变及其意义。用免疫组织化学显示神经丝蛋白和髓鞘碱性蛋白 ,观察人体脑干组织中的神经轴突及髓鞘在损伤后的病理学改变。在脑干损伤组 ,神经轴突在伤后 0 5h即有不规则肿胀及断裂 ,神经髓鞘在伤后 19～ 2 2h有明显曲折、不完全剥脱及节段性消失 ;而在正常脑干组 ,神经轴突和髓鞘均无明显病理改变。脑干组织中的神经轴突和髓鞘在损伤后将发生改变 ,并可用于脑干损伤的死后诊断

Immunohistochemical study on pathological changes of axon and myelin sheath in injured brainstem

To study the significance of the pathological changes of axon and myelin sheath in injured brainstem.The neurofilament and myelin basic protein was demonstrated immunohistochemically,and the pathological changes of axons and myelin sheathes in human brainstem following trauma were observed.In the injured brainstem,irregular swelling and disconnecting axons could be found as early as 0 5h after injury, while pathological changes in myelin sheaths such as tortuous change,partial peeling off from axon and intermittent disappearance could be observed at 19～22h after injury.However,in the normal brainstem,there were no evident changes in axons and myelin sheaths.Pathological changes of axon and myelin sheath in brainstem would occur after trauma,which could be used for postmortem diagnosis of brainstem injury.